Simple Plex Human GFAP Cartridge

Catalog #: SPCKB-HF-000970 Datasheet / COA / SDS

Simple Plex Human GFAP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-HF-000970
SPCKA-HF-000970
SPCKC-HF-000970

Control Products Available

898211: Simple Plex Control for Human GFAP

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human GFAP Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cerebrospinal Fluid (25 uL)

Sensitivity

14.4 pg/mL

Assay Range

72.1 - 110,000 pg/mL (Cerebrospinal Fluid)

Specificity

Natural and recombinant human GFAP.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human GFAP in cerebrospinal fluid (CSF)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	40	40
Mean (pg/mL)	603	27073	709	30982
Standard Deviation	18.7	1217	78.1	3591
CV%	3.1	4.5	11	11.6

Recovery

Recovery was not necessary as GFAP shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human GFAP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human GFAP Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GFAP

Glial Fibrillary Acidic Protein (GFAP) is a type III intermediate filament protein. GFAP is the predominant component of astrocyte intermediate filaments in the central nervous system and is often used as an astrocytic marker. It has also been detected in the glial cells of the enteric nervous system and some Schwann cells in the peripheral nervous system.

Long Name:

Glial Fibrillary Acidic Protein

Entrez Gene IDs:

2670 (Human); 14580 (Mouse); 24387 (Rat)

Alternate Names:

ALXDRD; FLJ45472; GFAP astrocytes; GFAP; glial fibrillary acidic protein