Simple Plex Human GFAP (2nd Gen) Assay Cartridge

Catalog #: SPCKB-PS-009134 Datasheet / COA / SDS
Simple Plex Human GFAP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-009134
SPCKC-PS-009134
SPCKA-PS-009134
Control Products Available
Human GFAP Ella Assay
3 Images
Product Details
FAQs
Supplemental Products
Reviews

Simple Plex Human GFAP (2nd Gen) Assay Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (25 uL)
Sensitivity
0.64 pg/mL
Assay Range
2.5 - 9,600 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Specificity
This assay recognizes natural and recombinant human GFAP. 
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Glial Fibrillary Acidic Protein (GFAP) in cell culture supernates (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 62 59
Mean (pg/mL) 75.1 3705 76.3 3529
Standard Deviation 3.54 218 6.08 257
CV% 4.7 5.9 8 7.3

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 110 110-111
EDTA Plasma (n=4) 94 90-98
Heparin Plasma (n=4) 95 90-102
Serum (n=4) 91 86-100

Linearity

Samples containing and/or spiked with high concentrations of human GFAP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Human GFAP Ella Assay Linearity

Scientific Data

Human GFAP Ella Assay Standard Curve

Product Datasheets

You must select a language.

x

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GFAP

Glial Fibrillary Acidic Protein (GFAP) is a type III intermediate filament protein. GFAP is the predominant component of astrocyte intermediate filaments in the central nervous system and is often used as an astrocytic marker. It has also been detected in the glial cells of the enteric nervous system and some Schwann cells in the peripheral nervous system.

Long Name:
Glial Fibrillary Acidic Protein
Entrez Gene IDs:
2670 (Human); 14580 (Mouse); 24387 (Rat)
Alternate Names:
ALXDRD; FLJ45472; GFAP astrocytes; GFAP; glial fibrillary acidic protein

FAQs

No product specific FAQs exist for this product, however you may

View all Simple Plex Assays FAQs
Loading...

Reviews for Simple Plex Human GFAP (2nd Gen) Assay Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Human GFAP (2nd Gen) Assay Cartridge and earn rewards!

Have you used Simple Plex Human GFAP (2nd Gen) Assay Cartridge?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review