Simple Plex Human GFAP (2nd Gen) Assay Cartridge

Catalog #: SPCKB-PS-009134 Datasheet / COA / SDS

Simple Plex Human GFAP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-009134
SPCKC-PS-009134
SPCKA-PS-009134
ST01B-PS-009134

Control Products Available

897034: Simple Plex Control for Human GFAP 2nd Gen

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All GFAP Simple Plex Assays

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human GFAP (2nd Gen) Assay Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (25 uL)

Sensitivity

0.64 pg/mL

Assay Range

2.5 - 9,600 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Specificity

This assay recognizes natural and recombinant human GFAP.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Glial Fibrillary Acidic Protein (GFAP) in cell culture supernates (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF).

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	110	110-111
EDTA Plasma (n=4)	94	90-98
Heparin Plasma (n=4)	95	90-102
Serum (n=4)	91	86-100

Linearity

Samples containing and/or spiked with high concentrations of human GFAP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Human GFAP Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GFAP

Glial Fibrillary Acidic Protein (GFAP) is a type III intermediate filament protein. GFAP is the predominant component of astrocyte intermediate filaments in the central nervous system and is often used as an astrocytic marker. It has also been detected in the glial cells of the enteric nervous system and some Schwann cells in the peripheral nervous system.

Long Name:

Glial Fibrillary Acidic Protein

Entrez Gene IDs:

2670 (Human); 14580 (Mouse); 24387 (Rat)

Alternate Names:

ALXDRD; FLJ45472; GFAP astrocytes; GFAP; glial fibrillary acidic protein