Simple Plex Human CXCL5/ENA-78 Cartridge

Catalog #: SPCKB-PS-000334 Datasheet / COA / SDS

Simple Plex Human CXCL5/ENA-78 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000334
SPCKA-PS-000334
SPCKC-PS-000334

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898253: Simple Plex Control for Human CXCL5/ENA-78

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human CXCL5/ENA-78 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sensitivity

0.613 pg/mL

Assay Range

0.4 - 4,160 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human ENA-78.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human ENA-78 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

Recovery

Recovery was not necessary as ENA-78 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human ENA-78 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human ENA-78 Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL5/ENA-78

CXCL5/Epithelial Cell-derived Neutrophil-activating Peptide (ENA-78), is a member of the CXC subfamily of chemokines. Full-length CXCL5/ENA-78 is 114 amino acids (aa) in length with a predicted molecular weight of 12 kDa. Following the removal of the signal peptide, bioactive CXCL5/ENA-78 is 78 aa in length. CXCL5/ENA-78 can be N-terminally cleaved by Cathepsin G and Chymotrypsin to CXCL5/ENA-74 (74 aa) and CXCL5/ENA-70 (70 aa), which show increased potency relative to CXCL5/ENA-78. While murine LIX was thought to be an ortholog to human CXCL5/ENA-78, genome-wide analysis and a consensus in the field suggests that human CXCL5/ENA-78 does not have a true murine ortholog. CXCL5/ENA-78 is upregulated at sites of inflammation and is expressed by multiple hematopoietic cell types, fibroblasts, endothelial cells, vascular smooth muscle cells, and adipocytes. It acts as a chemoattractant for neutrophils, has angiogenic properties, and contributes to cancer progression.

Entrez Gene IDs:

6374 (Human)

Alternate Names:

chemokine (C-C motif) ligand 5; C-X-C motif chemokine 5; CXCL5; CXCL5/ENA-78; ENA78; ENA-78; ENA-78(1-78); Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; SCYB5ENA78