Simple Plex Human CCL26/Eotaxin-3 Cartridge

Catalog #: SPCKB-PS-000486 Datasheet / COA / SDS

Simple Plex Human CCL26/Eotaxin-3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD28, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000486
SPCKA-PS-000486
SPCKC-PS-000486

Control Products Available

898540: Simple Plex Control for Human CCL26/Eotaxin-3

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Related Research Areas

Simple Plex Human CCL26/Eotaxin-3 Cartridge Summary

Sample Type & Volume Required Per Well*

Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.17 pg/mL

Assay Range

0.4 - 3,400 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Eotaxin-3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Eotaxin-3 in serum and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	38	38
Mean (pg/mL)	20.2	959	21.2	952
Standard Deviation	1.17	85.3	2.06	101
CV%	5.8	8.9	9.7	10.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	108	103-113
Heparin Plasma (n=4)	108	99-112
Serum (n=4)	105	85-119

Linearity

Samples containing and/or spiked with high concentrations of human Eotaxin-3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay