Simple Plex Human ASC Cartridge

Catalog #: SPCKB-PS-000834 Datasheet / COA / SDS

Simple Plex Human ASC assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000834
SPCKA-PS-000834
SPCKC-PS-000834

Control Products Available

898571: Simple Plex Control for Human ASC

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All ASC Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human ASC Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

1.95 pg/mL

Assay Range

5.2 - 8,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human ASC.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human ASC in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	57	55
Mean (pg/mL)	65.2	3191	61.4	3062
Standard Deviation	3.21	170	6.23	294
CV%	4.9	5.3	10.1	9.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Linearity

Samples containing and/or spiked with high concentrations of human ASC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human ASC Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ASC

ASC contains an N-terminal Pyrin domain and C-terminal CARD (Caspase-recruitment Domain), and is a p53 target protein that serves as an adaptor to the pro-apoptotic Bax molecule. The interaction of ASC with Bax promotes apoptosis in response to many apoptotic stimuli. Methylation of the ASC gene has been correlated with decreased protein expression in numerous types of cancer.

Long Name:

Apoptosis-Associated Speck-Like Protein Containing A CARD

Entrez Gene IDs:

29108 (Human)

Alternate Names:

apoptosis-associated speck-like protein containing a CARD; ASC; CARD5; caspase recruitment domain protein 5; Caspase recruitment domain-containing protein 5; hASC; PYCARD; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1; TMS; TMS1; TMS-1