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Simple Plex Cytokine Screening Panel 2

Catalog #: ST01E-PS-005711 Datasheet / COA / SDS
Simple Plex 32x6 panel containing IL-10, IL-2, IFN-gamma 3rd Gen, TNF-alpha 2nd Gen, IL-8/CXCL8, and IL-6 2nd Gen with sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum)
Catalog # Availability Size / Price Qty
ST01E-PS-005711
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Summary Product Datasheets

Simple Plex Cytokine Screening Panel 2 Summary

The Simple Plex Human Multianalyte Assay is for the detection of human IFNg 3rd gen, IL-10, IL-2, IL-6 2nd gen, IL-8, and TNF-a 2nd gen in serum and plasma (EDTA/Heparin).

 
Sample Type & Volume Required Per Well Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity IFNg 0.17 pg/mL; IL-10 0.58 pg/mL; IL-2 0.54 pg/mL; IL-6 0.28 pg/mL; IL-8 0.19 pg/mL; TNF-a 0.3 pg/mL
Assay Range IFNg 0.17 - 4,000 pg/mL; IL-10 0.58 - 2,212 pg/mL; IL-2 0.54 - 2,050 pg/mL; IL-6 0.28 - 2,652 pg/mL; IL-8 0.19 - 1,804 pg/mL; TNF-a 0.3 - 1,160 pg/mL

Product Specifications

Reactivity Human
Specificity Natural and recombinant human IFNg 3rd gen, IL-10, IL-2, IL-6 2nd gen, IL-8, TNF-a 2nd gen.
Cross-reactivity <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested.
Interference No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

    Intra-Assay Precision Inter-Assay Precision
  Sample 1 2 1 2
IFN-gamma n 16 16 16 16
Mean (pg/mL) 21.1 1175 23.4 1192
Standard Deviation 1.48 33.4 2.52 99.4
CV (%) 7 2.8 10.8 8.3
IL-10 n 16 16 16 16
Mean (pg/mL) 33 1591 33.2 1681
Standard Deviation 1.55 95.1 2.43 120
CV (%) 4.6 6 7.1 7.1
IL-2 n 16 16 16 16
Mean (pg/mL) 15 648 13 608
Standard Deviation 0.8 32.7 1.13 34
CV (%) 5.3 5.1 8.7 5.6
IL-6 2nd gen n 16 16 16 16
Mean (pg/mL) 37.9 1763 38.7 1709
Standard Deviation 0.804 108 3.95 163
CV (%) 2.1 6.1 10.2 9.6
IL-8 n 16 16 16 16
Mean (pg/mL) 28.7 1362 27.3 1463
Standard Deviation 1.41 89.8 1.41 125
CV (%) 4.9 6.6 5.2 8.6
TNF alpha 2nd Gen n 16 16 16 16
Mean (pg/mL) 6.99 383 0.662 36.9
Standard Deviation 0.345 20.6 7.81 408
CV (%) 4.9 5.4 8.5 9

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

  Sample Type Average % Recovery Range %
IFN-gamma EDTA Plasma (n=4) 89 88-90
Heparin Plasma (n=4) 90 83-99
Serum (n=4) 95 83-102
IL-10 EDTA Plasma (n=6) 105 88-113
Heparin Plasma (n=6) 102 89-118
Serum (n=5) 94 88-98
IL-2 EDTA Plasma (n=4) 86 69-100
Heparin Plasma (n=4) 90 70-103
Serum (n=4) 92 69-102
IL-6 2nd gen EDTA Plasma (n=6) 95 92-98
Heparin Plasma (n=6) 93 91-94
Serum (n=6) 94 86-98
IL-8 EDTA Plasma (n=5) 89 75-102
Heparin Plasma (n=5) 95 88-102
Serum (n=3) 92 88-97
TNF alpha 2nd Gen EDTA Plasma (n=4) 97 89-105
Heparin Plasma (n=4) 89 77-96
Serum (n=4) 99 92-108

Specifications

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Applications
Simple Plex Automated Immunoassay

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