Antibodies for detecting SARS-CoV-2: R&D Systems

R&D Systems offers a range of antibodies that recognize and bind various SARS-CoV-2 antigens, such as the S1 and S2 subunits, as well as the receptor binding domain (RBD). These antibodies are highly validated and can be used to detect SARS-CoV-2 in multiple sample types by a variety of applications including ELISA, flow cytometry, and immunohistochemistry. Explore our range of SARS-CoV-2 antibodies and learn more about a specific antibody by clicking its catalog number.

Explore SARS-CoV-2 antibodies for:

ELISA Development
Flow Cytometry

Immunohistochemistry (IHC)
Western Blot

Additionally, visit our main Antibodies for Coronavirus Research page for more information on our antibodies and related resources for SARS-CoV-2 research.

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SARS-CoV-2 Matched Antibody Pairs for ELISA Development

Using ELISAs to detect SARS-CoV-2 antigens allows for the detection and quantification of the viral proteins in samples. R&D Systems offers SARS-CoV-2 antibodies for researchers who want to develop their own ELISAs. Our ELISA-validated antibodies have been tested for SARS-CoV-2 antigen detection using a sandwich immunoassay, allowing us to recommend a matched antibody pair for assay development.

R&D Systems™ Anti-SARS-CoV-2 Antibodies for ELISA
SARS-CoV-2 Antigen	Clone	Catalog #	ELISA Function	Catalog # of Paired ELISA Antibody
SARS-CoV-2 Spike RBD	1035240	MAB105406	Detection Antibody	MAB10580
	1035414	MAB10580	Capture Antibody	MAB105406
	1035709	MAB105806	Capture Antibody	MAB105807
	1035709	MAB105806	Detection Antibody	MAB105808
	1035740	MAB105807	Detection Antibody	MAB105806
	1035740	MAB105807	Detection Antibody	MAB105808
	1035753	MAB105808	Capture Antibody	MAB105807
	1035753	MAB105808	Capture Antibody	MAB105806
SARS-CoV-2 Nucleocapsid	1035143	MAB104742	Detection Antibody	MAB104741
SARS-CoV-2 Nucleocapsid	1035145	MAB104741	Capture Antibody	MAB104742

Detection of SARS-CoV-2 Spike RBD by ELISA. Recombinant SARS-CoV-2 Spike RBD protein was serially diluted 2-fold and captured by a Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (Catalog # MAB10580) coated on a Clear Polystyrene Microplate (Catalog # DY990). A different Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (Catalog # MAB105406) was biotinylated and used to detect the captured SARS-CoV-2 Spike RBD protein. Detection of the standard curve was achieved by adding Streptavidin-HRP (Catalog # DY998) to the plate, followed by a Substrate Solution (Catalog # DY999) and then a Stop Solution (Catalog # DY994) to stop the enzymatic reaction.

Detection of SARS-CoV-2 Spike S2 Subunit and SARS-CoV-2 Spike trimer by Western Blot.

SARS-CoV-2 Antibodies for Flow Cytometry

Detection of SARS-CoV-2 antigens by flow cytometry offers a range of uses for SARS-CoV-2 research and COVID-19 therapy development. Flow detection of the viral antigens in concert with live/dead cell stains or other conjugated antibodies, such as those for cellular markers, allows for the characterization of infected cells as well as screening potential therapeutics for antibody binding to infected cells. R&D Systems offers SARS-CoV-2 antibodies validated for flow cytometry for use in SARS-CoV-2 research and therapy development.

R&D Systems™ Anti-SARS-CoV-2 Antibodies for Flow Cytometry Staining
SARS-CoV-2 Antigen	Clone	Catalog #	Conjugate
SARS-CoV-2 Spike S1 Subunit	1035206	MAB105403	Unconjugated
		FAB105403G	Alexa Fluor^® 488
		FAB105403N	Alexa Fluor 700
		FAB105403R	Alexa Fluor 647
		FAB105403S	Alexa Fluor 750
		FAB105403T	Alexa Fluor 594
		FAB105403U	Alexa Fluor 350
		FAB105403V	Alexa Fluor 405
	1035226	MAB105805	Unconjugated
		FAB105805G	Alexa Fluor^® 488
		FAB105805N	Alexa Fluor 700
		FAB105805R	Alexa Fluor 647
		FAB105805S	Alexa Fluor 750
		FAB105805T	Alexa Fluor 594
		FAB105805U	Alexa Fluor 350
		FAB105805V	Alexa Fluor 405

Detection of SARS-CoV-2 Spike Protein by Flow Cytometry. HEK293 human embryonic kidney cell line, transfected with human ACE-2 and eGFP, was incubated with Recombinant SARS-CoV-2 Spike S1 Subunit His-Tag Protein (Catalog # 10522-CV), then stained with a Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (A; Catalog # MAB105403) or a Mouse IgG1 Isotype Control Antibody (B; Catalog # MAB002), followed by an APC-Conjugated Goat Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). SARS-CoV-2 Spike protein, bound to ACE-2 expressed on the surface of the transfected HEK293 cells, was detected.

SARS-CoV-2 Spike Protein bound to ACE-2 on live, transfected HEK293 cells was detected by flow cytometry.

SARS-CoV-2 Antibodies for Immunohistochemistry (IHC)

Detection of SARS-CoV-2 by IHC is a vital tool for researching SARS-CoV-2. Visualization of the SARS-CoV-2 proteins within cells and tissues by IHC allows for the semiquantitative identification of the virus while maintaining the spatial context of its expression, such as cell types targeted by SARS-CoV-2. R&D Systems offers SARS-CoV-2 antibodies validated for IHC for characterizing SARS-CoV-2 infection.

R&D Systems Anti-SARS-CoV-2 Antibodies for IHC Staining
SARS-CoV-2 Antigen	Clone	Catalog #
SARS-CoV-2 Spike RBD	1035423	MAB105802
SARS-CoV-2 Spike S1 Subunit	1035206	MAB105403
SARS-CoV-2 Spike S1 Subunit	2811A	MAB105407
SARS-CoV-2 Nucleocapsid	1035111	MAB10474
SARS-CoV-2 Nucleocapsid	1035145	MAB104741

Spike RBD in SARS-CoV-2 Infected Human Lung. Spike RBD was detected in immersion-fixed, paraffin-embedded sections of SARS-CoV-2 infected human lung tissue using a Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (Catalog # MAB105802), followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Prior to incubation with the primary antibody, the tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). The tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.

Spike RBD in human lung tissue infected with SARS-CoV-2 detected with immunohistochemistry.

SARS-CoV-2 Antibodies for Western Blot

Immunoblotting is an invaluable tool for COVID-19 research. Investigating mechanisms that allow SARS-CoV-2 to infect cells and escape immune surveillance while maintaining high infectivity often utilizes in vitro cell models with western blot analysis of SARS-CoV-2 proteins to confirm SARS-CoV-2 infection. Western blot is also used to determine which protein antigens are recognized by potential therapies such as antibody-based vaccines or sera from convalescent patients. R&D Systems offers SARS-CoV-2 antibodies validated for western blot for use in SARS-CoV-2 research and therapy development.

R&D Systems Anti-SARS-CoV-2 Antibodies for Western Blot Detection
SARS-CoV-2 Antigen	Clone	Catalog #
SARS-CoV-2 Spike RBD	1034522	MAB10540
SARS-CoV-2 Spike RBD	1034515	MAB105401
SARS-CoV-2 Spike S1 Subunit	1035206	MAB105403
	2811A	MAB105407
	1035341	MAB10637
SARS-CoV-2 Spike S2 Subunit	1034617	MAB10557
SARS-CoV-2 Spike S2 Subunit	1037111	MAB106371
SARS-CoV-2 Nucleocapsid	1035111	MAB10474
	1035145	MAB104741
	1035143	MAB104742