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SANT-1

Catalog #: 1974
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1974/10
1974/50
SANT-1 | CAS No. 304909-07-7 | Hedgehog Signaling Inhibitors
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Description: Inhibitor of hedgehog (Hh) signaling; antagonizes smoothened activity

Chemical Name: N-[(3,5-dimethyl-1-phenyl-1H-pyrazol-4-yl)methylene]-4-(phenylmethyl)-1-piperazinamine

Purity: ≥98%

Product Details
Citations (10)
Supplemental Products
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Biological Activity Technical Data Background Product Datasheets Calculators

Biological Activity

SANT-1 is a potent, cell-permeable inhibitor of Sonic hedgehog (Shh) signaling; high affinity antagonist of smoothened activity (KD = 1.2 nM). Inhibits smoothened agonist effects with an IC50 of 20 nM (in Shh-LIGHT2 cells). Has been used in protocols to generate pancreatic β cells from hPSCs.

For more information about how SANT-1 may be used, see our protocol: Generation of β cells from hPSCs

Technical Data

M.Wt:
373.5
Formula:
C23H27N5
Solubility:
Soluble to 50 mM in DMSO
Purity:
≥98%
Storage:
Store at +4°C
CAS No:
304909-07-7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Lmx1a allows context-specific isolation of progenitors of GABAergic or dopaminergic neurons during neural differentiation of embryonic stem cells.
    Nefzger CM, Su CT, Fabb SA, Hartley BJ, Beh SJ, Zeng WR, Haynes JM, Pouton CW
    Stem Cells, 2012;30(7):1349-61.
  2. Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors
    KF Cogger, A Sinha, F Sarangi, EC McGaugh, D Saunders, C Dorrell, S Mejia-Guer, Y Aghazadeh, JL Rourke, RA Screaton, M Grompe, PR Streeter, AC Powers, M Brissova, T Kislinger, MC Nostro
    Nat Commun, 2017;8(1):331.
  3. Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines.
    Nostro M, Sarangi F, Yang C, Holland A, Elefanty A, Stanley E, Greiner D, Keller G
    Stem Cell Reports, 2015;4(4):591-604.
  4. Small molecule modulation of smoothened activity.
    Chen et al.
    Proc.Natl.Acad.Sci., 2002;99:14071
  5. Stem-Cell-Derived beta-Like Cells with a Functional PTPN2 Knockout Display Increased Immunogenicity
    TM Triolo, JQ Matuschek, R Castro-Gut, AH Shilleh, SPM Williams, MS Hansen, K McDaniel, JM Barra, A Michels, HA Russ
    Cells, 2022;11(23):.

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Citations for SANT-1

The citations listed below are publications that use Tocris products. Selected citations for SANT-1 include:

10 Citations: Showing 1 - 10

  1. Sufu- And Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals.
    Authors: Yung Et al.
    Nat.Commun.  2019;10:4647
  2. Generation of hepatocyte- and endocrine pancreatic-like cells from human induced endodermal progenitor cells.
    Authors: Sambathkumar Et al.
    PLoS One  2018;13:e0197046
  3. Phosphorylation of NEUROG3 Links Endocrine Differentiation to the Cell Cycle in Pancreatic Progenitors.
    Authors: Krentz Et al.
    Dev Cell  2017;41:129
  4. Hedgehog associated to microparticles inhibits adipocyte differentiation via a non-canonical pathway.
    Authors: Fleury Et al.
    Sci Rep  2016;6:23479
  5. Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiation.
    Authors: Yuan Et al.
    Nat Commun  2016;21:11024
  6. Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines.
    Authors: Nostro Et al.
    Neuropsychopharmacology  2015;4:591
  7. MRT-92 inhibits Hedgehog signaling by blocking overlapping binding sites in the transmembrane domain of the Smoothened receptor.
    Authors: Hoch Et al.
    FASEB J  2015;29:1817
  8. Itraconazole inhibits enterovirus replication by targeting the oxysterol-binding protein.
    Authors: Strating Et al.
    Cell Rep  2015;10:600
  9. GLI activation by atypical protein kinase C Ι/λ regulates the growth of basal cell carcinomas.
    Authors: Atwood Et al.
    Stem Cell Reports  2013;494:484
  10. Glucocorticoid compounds modify smoothened localization and hedgehog pathway activity.
    Authors: Wang Et al.
    Chem Biol  2012;19:972

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