Blocking & Neutralizing Antibodies: R&D Systems

Blocking and neutralizing antibodies validated in-house

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With over 1,400 antibodies that have been validated, our selection of neutralization and blocking antibodies is unmatched. Our commitment to quality antibodies allows you to be confident in your results and help you generate publication-quality data.

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Neutralization Antibody Validation and Quality

Tested in Relevant Bioassays

By utilizing our selection of in-house manufactured proteins, we are able to leverage our bioassay expertise to ensure that our antibodies block/neutralize the appropriate cellular function. Our assays include proliferation, chemotaxis, protein secretion, adhesion, and in vitro enzyme neutralization. We continuously add new assays to ensure the highest specificity based on the latest research data.

Low Endotoxin Levels

Our neutralizing antibodies are manufactured to ensure low endotoxin levels. Each lot is QC tested for the level of endotoxin and the information is reported on each individual datasheet. You won’t have to worry about endotoxin-induced nonspecific cellular responses.

What Is The Difference Between Blocking And Neutralizing Antibodies?

A blocking antibody binds its target and directly interferes with its function, such as blocking cell adhesion or receptor-ligand binding. A neutralizing antibody binds its target and negates its downstream cellular effects, such as cell proliferation or chemotaxis.

Cell Adhesion Mediated by Osteopontin/OPN and Neutralization by Human Osteopontin/OPN Antibody. Recombinant Human Osteopontin/OPN (Catalog # 1433-OP), immobilized onto a microplate, supports the adhesion of the HEK293 human embryonic kidney cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Osteopontin/OPN (1 µg/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human Osteopontin/OPN Monoclonal Antibody (Catalog # MAB14334). The ND50 is typically 0.5-1.5 µg/mL.

OPN blocking antibody blocks recombinant OPN in an adhesion assay.

Chemotaxis Induced by CXCL13/BLC/BCA-1 and Neutralization by Human CXCL13/BLC/BCA-1 Antibody. Recombinant Human CXCL13/BLC/BCA-1 (Catalog # 801-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL13/BLC/BCA-1 (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL13/BLC/BCA-1 Monoclonal Antibody (Catalog # MAB8012). The ND50is typically 0.5-2.5 µg/mL.

CXCL13 neutralizing antibody blocks recombinant CXCL13 in a chemotaxis assay.

Custom Antibody Services

Unable to find a neutralizing antibody for your research? Utilize our scientific expertise in antibody engineering and development. We have the ability to generate new antibodies by using our full length recombinant proteins as well as offer panels of antibodies from our hybridomas so you can screen multiple clones at once. By having the ability to create recombinant antibodies in-house, we can also immortalize your antibody by converting your existing clone to a recombinant antibody or modifying your current antibody to meet your needs.

Learn About Our Custom Antibody Services

Take advantage of our ability to perform blocking and neutralizing bioassays in-house. Our scientists in Bioassay Services will work with you to determine and optimize an assay catered to your needs. You will be provided with raw data and a professionally formatted report with a detailed summary of the bioassay protocol that was used.

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Blocking and Neutralizing Antibody Research Areas

R&D Systems offers a wide selection of blocking and neutralizing antibodies across many different research areas. Some research areas include Immune Checkpoint Blockade, Chemokine Functions, Angiogenic Factors, and Developmental Factors. Select one of our top targets below, and view our available products.

Block Your Chemokine Function Antibodies