Recombinant SARS-CoV Nucleocapsid His-tag Protein, CF

Catalog #: 10710-CV Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10710-CV-100

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant SARS-CoV Nucleocapsid His-tag Protein, CF Summary

Product Specifications

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived sars-cov Nucleocapsid protein
Met1-Ala422, with a C-terminal 6-His tag

Accession #

NP_828858.1

N-terminal Sequence
Analysis

Ser2, determined by protein ID

Predicted Molecular Mass

47 kDa

SDS-PAGE

41-55 kDa, under reducing conditions

Product Datasheets

10710-CV

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10710-CV

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with Trehalose.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Nucleocapsid

SARS-CoV was discovered in association with cases of severe acute respiratory syndrome (SARS) that infected more than 8,000 persons with over 900 fatalities worldwide in 2002-2003 (1). It belongs to a family of viruses known as coronaviruses that also include MERS and SARS-Cov2 that causes the global pandemic coronavirus disease 2019 (Covid-19). Coronavirus is commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). While the S, E and M proteins build up the viral envelop, the N protein is involved in transcription, replication, and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (2, 3). The SARS-CoV N protein is a ~45 kDa protein composed of two independent structural domains connected by a linker region. Both the N-terminal and the linker regions contain RNA binding domains, while the C-terminal region is responsible for the oligomerization of the N protein (2, 3). The SARS-CoV N protein shares 90% amino acid sequence identity with SARS-CoV-2 N protein. The N protein is an abundant protein during coronavirus infection and displays high immunogenic activity (4, 5), so it has been used to develop serological diagnostic kit for SARS IgM and IgG antibody tests.

References