Recombinant SARS-CoV-2 Spike S2 Subunit His-tag Protein, CF
Recombinant SARS-CoV-2 Spike S2 Subunit His-tag Protein, CF Summary
Product Specifications
Ser686-Lys1211, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10594-CV
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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2 μg/lane of Recombinant SARS-CoV-2 Spike S2 Subunit His-tag (Catalog # 10594-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 80-100 kDa.
Reconstitution Calculator
Background: Spike S2 Subunit
SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that also include MERS and SARS-CoV-1. Coronaviruses are commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) (1). SARS-CoV-2 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). As with most coronaviruses, proteolytic cleavage of the S protein into two distinct peptides, S1 and S2 subunits, is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (2-4). A metallopeptidase, angiotensin-converting enzyme 2 (ACE-2), has been identified as a functional receptor for SARS-CoV2, similar to SARS-CoV-1, through interaction with a receptor binding domain (RBD) located at the C-terminus of S1 subunit (5, 6). The S2 subunit of SARS-CoV-2 shares 90% and 41% amino acid sequence identity with the S2 subunit of SARS-CoV-1 and MERS, respectively. It has been demonstrated the S Protein can invade host cells through the CD147/EMMPRIN receptor and mediate membrane fusion (7, 8).
- Rota, P.A. et al. (2003) Science 300:1394.
- Bosch, B.J. et al. (2003). J. Virol. 77:8801.
- Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. USA 106:5871.
- Millet, J.K. and G. R. Whittaker (2015) Virus Res. 202:120.
- Li, W. et al. (2003) Nature 426:450.
- Wong, S.K. et al. (2004) J. Biol. Chem. 279:3197.
- Wang, X. et al. (2020) https://doi.org/10.1038/s41423-020-0424-9.
- Wang, K. et al. (2020) bioRxiv https://www.biorxiv.org/content/10.1101/2020.03.14.988345v.
Citations for Recombinant SARS-CoV-2 Spike S2 Subunit His-tag Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Class switch towards spike protein-specific IgG4 antibodies after SARS-CoV-2 mRNA vaccination depends on prior infection history
Authors: Kiszel, P;Sík, P;Miklós, J;Kajdácsi, E;Sinkovits, G;Cervenak, L;Prohászka, Z;
Scientific reports
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Capture -
Evidence, detailed characterization and clinical context of complement activation in acute multisystem inflammatory syndrome in children
Authors: G Sinkovits, J Schnur, L Hurler, P Kiszel, ZZ Prohászka, P Sík, E Kajdácsi, L Cervenak, V Maráczi, M Dávid, B Zsigmond, É Rimanóczy, C Bereczki, L Willems, EJM Toonen, Z Prohászka
Scientific Reports, 2022-11-17;12(1):19759.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Standard -
Children develop robust and sustained cross-reactive spike-specific immune responses to SARS-CoV-2 infection
Authors: AC Dowell, MS Butler, E Jinks, G Tut, T Lancaster, P Sylla, J Begum, R Bruton, H Pearce, K Verma, N Logan, G Tyson, E Spalkova, S Margielews, GS Taylor, E Syrimi, F Baawuah, J Beckmann, IO Okike, S Ahmad, J Garstang, AJ Brent, B Brent, G Ireland, F Aiano, Z Amin-Chowd, S Jones, R Borrow, E Linley, J Wright, R Azad, D Waiblinger, C Davis, EC Thomson, M Palmarini, BJ Willett, WS Barclay, J Poh, G Amirthalin, KE Brown, ME Ramsay, J Zuo, P Moss, S Ladhani
Nature Immunology, 2021-12-22;23(1):40-49.
Species: Human
Sample Types: Plasma
Applications: Neutralization -
Humoral immune responses during SARS-CoV-2 mRNA vaccine administration in seropositive and seronegative individuals
Authors: E Fraley, C LeMaster, E Geanes, D Banerjee, S Khanal, E Grundberg, R Selvaranga, T Bradley
Bmc Medicine, 2021-07-26;19(1):169.
Species: Human
Sample Types: Plasma
Applications: Bioassay
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Average Rating: 5 (Based on 1 Review)
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Reason for Rating: Worked great for in vitro testing against THP-1 cells.
