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Recombinant SARS-CoV-2 Membrane Fc Chimera Protein, CF

Catalog #: 10690-CV
1 Citation Datasheet / COA / SDS
Extracellular Domain
Catalog # Availability Size / Price Qty
10690-CV-100
Recombinant SARS-CoV-2 Membrane Fc Chimera Protein SDS-PAGE.
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Product Details
Citations (1)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant SARS-CoV-2 Membrane Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Chinese Hamster Ovary cell line, CHO-derived sars-cov-2 Membrane protein
SARS-CoV-2 Membrane Protein 
(Met1-Asn21)
Accession # YP_009724393.1		IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
YP_009724393.1
N-terminal Sequence
Analysis
Met1
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
29 kDa
SDS-PAGE
33-43 kDa, under reducing conditions

Product Datasheets

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10690-CV

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10690-CV

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE View Larger

2 μg/lane of Recombinant SARS-CoV-2 Membrane Fc Chimera Protein (Catalog # 10690-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions. Visualized by Coomassie® Blue staining, showing bands at 33-43 kDa and 60-80 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Membrane

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). M protein is the most abundant structural protein in the coronavirus membrane, and it is predicted to span the membrane three times, with a short N-terminal domain outside the viral envelope and a long C-terminal domain inside the virion (2). SARS-CoV-2 M protein is a 222 amino acid (aa) glycoprotein that is composed of an 18 aa N-terminal domain on the viral surface, 3 transmembrane domains, and a 122 aa C-terminal domain inside the viral envelope. SARS-CoV-2 M protein shares 89.14%, 98.6%, 98.2%, and 38.36% aa similarity with SARS-CoV-1, bat SARS-CoV, pangolin SARS-CoV, and MERS-CoV M proteins, respectively (3). The M protein of coronavirus plays an important role in assembly of viral particles by interacting with other structural proteins, especially with the E protein (4, 5). In SARS-CoV-2, M protein, combined with E protein, regulates intracellular trafficking of the S Protein and its unique furin-mediated processing (6).

References
  1. Wu, F. et al. (2020) Nature 579:265.
  2. Mousavizadeh, L. and S. Ghasemi (2020) J. Microbiol. Immunol. Infect. doi:10.1016/j.jmii.2020.03.022.
  3. Thomas, S. (2020) Pathog. Immun. 5:342.
  4. Masters, P.S. (2006) Adv. Virus. Res. 66:193.
  5. Corse, E. and C.E. Machamer (2003) Virology 312:25.
  6. Boson, B. et al. (2020) bioRxiv doi:10.1101/2020.08.24.260901.
Long Name
Membrane Glycoprotein
Entrez Gene IDs
43740571 (SARS-CoV-2)
Alternate Names
M protein; Membrane

Citation for Recombinant SARS-CoV-2 Membrane Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. ACE2-dependent and -independent SARS-CoV-2 entries dictate viral replication and inflammatory response during infection
    Authors: Duan, T;Xing, C;Chu, J;Deng, X;Du, Y;Liu, X;Hu, Y;Qian, C;Yin, B;Wang, HY;Wang, RF;
    Nature cell biology
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay

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