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Recombinant SARS-CoV-2 3CL Protease, CF

Catalog #: E-720
2 Citations Datasheet / COA / SDS
E. coli Expressed, Bioactivity Tested
Catalog # Availability Size / Price Qty
E-720-050
Recombinant SARS-CoV-2 3CL Protease Inhibition Activity
3 Images
Product Details
Citations (2)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant SARS-CoV-2 3CL Protease, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant SARS-CoV-2 3CL Protease is a cysteine protease that cleaves proteins with sequences including LQ(S/A/G).  The cleavage occurs c-terminal to the glutamine residue.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial SARS-CoV-2 3CL Protease concentration of 20-40 nM when using substrate S-720.
Source
E. coli-derived sars-cov-2 3CL Protease protein
Ser1 - Gln306
Accession #
YP_009725301.1
Predicted Molecular Mass
34 kDa

Product Datasheets

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E-720

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-720

Formulation Supplied as a solution in HEPES, NaCl, DTT and EDTA.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Scientific Data

Inhibition Activity Recombinant SARS-CoV-2 3CL Protease Inhibition Activity View Larger

GC376 achieves 50% inhibition of 30 nM SARS-CoV-2 3CL Protease (E-720) at a concentration of 54-71 nM.

SDS-PAGE View Larger

SDS-PAGE of Recombinant SARS-CoV-2 3CL Protease. 1 μg and 2 μg of Recombinant SARS-CoV-2 3CL Protease (Catalog # E-720) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 34 kDa.

Mass Spectrometry View Larger

LC-MS analysis of SARS-CoV-2 3CL Protease. SARS-CoV-2 3CL Protease (Catalog # E-720) was analyzed by a Thermo Q Exactive HF instrument then deconvoluted using Thermo BioPharma Finder software. The expected monoisotopic mass is 33774.478 Da.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 3CL Protease

The 3CL protease (aka 3CLpro, Mpro or "Main" Protease) from the human SARS-CoV-2 coronavirus (Severe Acute Respiratory Syndrome coronavirus 2) is a C30-type cysteine protease located within the non-structural proteins 3 (NS3) section of the viral polypeptide.  3CLpro activity is required to process the viral polyprotein into functional, mature subunits, and there are 11 or more sites of cleavage, many containing the sequence LQ[S/A/G]; the protease cleaves c-terminal to the glutamine amino acid.  Along with the CoV-2 Papain-Like Protease (catalog number E-611), 3CLpro presents an attractive target for therapeutic intervention for COVID-19.  Because no human proteases with a similar cleavage specificity are known, inhibitors of 3CLpro are unlikely to cause mechanism-based toxicity.  

References
  1. Chen Y.W., et al. (2020) F1000Res. 9:129.
  2. Pillaiyar T., et al. (2016) J. Med. Chem.  59: 6595.
  3. Zhang L., et al. (2020) Science 368:409.
Long Name
3CL Protease
Alternate Names
3CL Protease; 3CL Proteinase; 3C-like main protease; 3CL-Mpro; COVID-19; M Proteinase; Main Protease; Mpro

Citations for Recombinant SARS-CoV-2 3CL Protease, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A Prototype Assay Multiplexing SARS-CoV-2 3CL-Protease and Angiotensin-Converting Enzyme 2 for Saliva-Based Diagnostics in COVID-19
    Authors: Suresh, V;Sheik, DA;Detomasi, TC;Zhao, T;Zepeda, T;Saladi, S;Rajesh, UC;Byers, K;Craik, CS;Davisson, VJ;
    Biosensors
    Applications: Bioassay
  2. DELTEX E3 ligases ubiquitylate ADP-ribosyl modification on protein substrates
    Authors: K Zhu, MJ Suskiewicz, A Hloušek-Ka, H Meudal, A Miko?, V Aucagne, D Ahel, I Ahel
    Science Advances, 2022-10-05;8(40):eadd4253.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

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