Recombinant Rhesus Macaque/Cynomolgus IFN-alpha Protein

Catalog #: 14110-1
2 Citations Datasheet / COA / SDS

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14110-1

R&D Systems Recombinant Proteins and Enzymes

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Citations (2)

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Recombinant Rhesus Macaque/Cynomolgus IFN-alpha Protein Summary

Product Specifications

Purity

>98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Interferon was titrated with the use of the cytopathic effect inhibition assay as listed. The protein has similar activity to Human IFN Alpha 2 on human, rhesus and bovine cells.

Bovine (MDBK/VSV) – performed as described [Rubinstein et al. (1981) J. Virol. 37(2):755] The EC₅₀ for interferon in this assay is ~5 U/ml.

Human (A549/EMCV) – performed as described [Budd et al. (1985) Canc. Chem. Pharm. 12:39]. The EC₅₀ for interferon in this assay is ~1 U/ml.

Source

E. coli-derived cynomolgus monkey IFN-alpha protein

Accession #

XM_001107516

Predicted Molecular Mass

19.5 kDa

Product Datasheets

14110-1

Product Datasheet

COA

SDS

14110-1

Formulation	Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Reconstitution Calculator

Background: IFN-alpha

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Long Name

Interferon alpha

Entrez Gene IDs

3440 (Human)

Alternate Names

IFNA; IFNA2; IFNA2b; IFNalpha; IFN-alpha; IFN-alphaA; INFA2; interferon alpha 2; LeIF D

Related Research Areas

Citations for Recombinant Rhesus Macaque/Cynomolgus IFN-alpha Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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A sensitive assay for measuring whole-blood responses to type I IFNs
Authors: Gervais, A;Le Floc'h, C;Le Voyer, T;Bizien, L;Bohlen, J;Celmeli, F;Al Qureshah, F;Masson, C;Rosain, J;Chbihi, M;Lévy, R;Castagnoli, R;Rothenbuhler, A;Jouanguy, E;Zhang, Q;Zhang, SY;Béziat, V;Bustamante, J;Puel, A;Bastard, P;Casanova, JL;
Proceedings of the National Academy of Sciences of the United States of America
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
First in human phase I trial of 852A, a novel systemic toll-like receptor 7 agonist, to activate innate immune responses in patients with advanced cancer.
Authors: Dudek AZ, Yunis C, Harrison LI, Kumar S, Hawkinson R, Cooley S, Vasilakos JP, Gorski KS, Miller JS
Clin. Cancer Res., 2007-12-01;13(23):7119-25.
Species: Human
Sample Types: Serum