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Recombinant Rat IGF-I/IGF-1 Protein, CF

Catalog #: 4326-RG
10 Citations Datasheet / COA / SDS
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4326-RG-050
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Citations (10)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat IGF-I/IGF-1 Protein, CF Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.6-3 ng/mL.
Source
E. coli-derived rat IGF-I/IGF-1 protein
MHHHHHHN Rat IGF-I/IGF-1
(Gly23-Ala92)
Accession # AAA41386
N-terminus C-terminus
Accession #
AAA41386
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
8.8 kDa

Product Datasheets

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4326-RG

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4326-RG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGF-I/IGF-1

Insulin-like growth factor I (IGF-I or IGF-1), also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Rat IGF-I/IGF-1 is synthesized as four precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I/IGF-1 is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature rat IGF-I/IGF-1 shares 96% and 99% aa sequence identity with human and mouse IGF-I/IGF-1, respectively (2, 3), and exhibits cross-species activity. It shares 61% aa sequence identity with mature rat IGF-II/IGF-2. Circulating IGF-I/IGF-1 is produced by hepatocytes, while local IGF-I/IGF-1 is produced by many other tissues in which it has paracrine effects (1). IGF-I/IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (4). IGF-I/IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (5 - 8). It plays an important role in muscle regeneration and tumor progression (1, 9). IGF-I/IGF-1 binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (10). Heterodimers of insulin receptor/IGF-I R also exist and respond to IGF-I/IGF-1 but not to insulin stimulation (11). IGF-I/IGF-1 association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (12).

References
  1. Philippou, A. et al. (2007) In Vivo 21:45.
  2. Roberts, Jr., C.T. et al. (1987) Mol. Endocrinol. 1:243.
  3. Shimatsu, A. and P. Rotwein (1987) J. Biol. Chem. 262:7894.
  4. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
  5. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
  6. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
  7. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
  8. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
  9. Samani, A.A. et al. (2007) Endocr. Rev. 28:20.
  10. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
  11. Benyoucef, S. et al. (2007) Biochem. J. 403:603.
  12. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Entrez Gene IDs
3479 (Human); 16000 (Mouse); 24482 (Rat)
Alternate Names
IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C

Citations for Recombinant Rat IGF-I/IGF-1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Degranulation of Murine Resident Cochlear Mast Cells: A Possible Factor Contributing to Cisplatin-Induced Ototoxicity and Neurotoxicity
    Authors: B Karayay, H Olze, AJ Szczepek
    International Journal of Molecular Sciences, 2023-02-27;24(5):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  2. Mast Cells in the Auditory Periphery of Rodents
    Authors: AJ Szczepek, T Dudnik, B Karayay, V Sergeeva, H Olze, A Smorodchen
    Brain Sci, 2020-10-01;10(10):.
    Species: Mouse, Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Serum IgG-induced microglial activation enhances neuronal cytolysis via the NO/sGC/PKG pathway in children with opsoclonus-myoclonus syndrome and neuroblastoma
    Authors: X Ding, W Yang, Q Ren, J Hu, S Yang, W Han, J Wang, X Wang, H Wang
    J Neuroinflammation, 2020-06-16;17(1):190.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Role of Programmed Cell Death 4 (PDCD4)-Mediated Akt Signaling Pathway in Vascular Endothelial Cell Injury Caused by Lower-Extremity Ischemia-Reperfusion in Rats
    Authors: H Chen, H Zhu, J Yang, Y Zhu, J Mei, H Shen, K Liang, X Zhang
    Med. Sci. Monit., 2019-06-29;25(0):4811-4818.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  5. Young plasma ameliorates aging-related acute brain injury after intracerebral hemorrhage
    Authors: JJ Yuan, Q Zhang, CX Gong, FX Wang, JC Huang, GQ Yang, L Liu, K Zhou, R Xu, Q Chen, Y Zhou, XY Xiong, QW Yang
    Biosci. Rep., 2019-05-17;0(0):.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  6. miR-129 controls axonal regeneration via regulating insulin-like growth factor-1 in peripheral nerve injury
    Authors: H Zhu, C Xue, M Yao, H Wang, P Zhang, T Qian, S Zhou, S Li, B Yu, Y Wang, X Gu
    Cell Death Dis, 2018-06-18;9(7):720.
    Species: Rat
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  7. Mechano-growth factor peptide, the COOH terminus of unprocessed insulin-like growth factor 1, has no apparent effect on myoblasts or primary muscle stem cells.
    Authors: Fornaro, Mara, Hinken, Aaron C, Needle, Saul, Hu, Erding, Trendelenburg, Anne-Ulr, Mayer, Angelika, Rosenstiel, Antonia, Chang, Calvin, Meier, Viktor, Billin, Andrew N, Becherer, J David, Brace, Arthur D, Evans, William, Glass, David J, Russell, Alan J
    Am J Physiol Endocrinol Metab, 2013-11-19;306(2):E150-6.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Insulin-like growth factor-1 lowers spreading depression susceptibility and reduces oxidative stress.
    J. Neurochem., 2012-05-17;122(1):221-9.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Age-related changes in rat bone-marrow mesenchymal stem cell plasticity.
    Authors: Asumda FZ, Chase PB
    BMC Cell Biol., 2011-10-12;12(0):44.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Histone deacetylase inhibition attenuates diabetes-associated kidney growth: potential role for epigenetic modification of the epidermal growth factor receptor.
    Authors: Gilbert RE, Huang Q, Thai K, Advani SL, Lee K, Yuen DA, Connelly KA, Advani A
    Kidney Int., 2011-03-09;79(12):1312-21.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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