Recombinant Rat CXCL3/CINC-2 alpha Protein

Recombinant Rat CXCL3/CINC-2 alpha Protein Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: CXCL3/GRO gamma/CINC-2/DCIP-1

The rat chemokines CINC (cytokine-induced neutrophil chemoattractant)-1, CINC-2 alpha, CINC-2 beta and CINC-3 (also named MIP-2) constitute a group of rat CXC chemokines that show significant sequence similarity to human GROs and mouse MIP-2 but not IL-8. CINC-2 alpha, CINC-2 beta were originally purified as novel neutrophil chemoattractants from the conditioned medium of rat granulation tissue which also contain CINC-1 and CINC-3. Based on amino acid (aa) sequence analysis of the purified CINC-2 alpha protein and sequencing of the CINC-2 beta cDNA clone, both mature CINC-2 alpha and CINC-2 beta were shown to contain 68 aa residues. The amino acid sequences of the two CINC-2 proteins are identical except for three carboxy-terminal residues. CINC-2 beta cDNA encodes a 100 aa residue precursor protein with a 32 aa residue signal peptide that is removed to yield the mature secreted protein. At the protein sequence level, mature CINC-2 proteins are 63% identical to CINC-1 and 80% identical to CINC-3. CINC-2 proteins represent the major chemokines purified from conditioned medium of granulation tissue or LPS-induced inflammatory exudate. Other cell types known to produce CINC-2 proteins include activated macrophages and fibroblasts.

Recombinant and natural CINC-2 proteins have been shown to be specific neutrophil chemoattractants both in vivo and in vitro. On the basis of cross-desensitization results of the various CINC proteins, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC-3-specific receptor as well as a second common receptor shared by all CINCs.

Related Research Areas

• Chemokines and Receptors in Angiogenesis
• CXC Chemokines and Receptors
• Inflammatory Mediators
• Macrophage Activation Markers