Recombinant Rat B7-H3 Fc Chimera Protein, CF Summary
Product Specifications
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10610-B3
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Scientific Data
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Recombinant Rat B7-H3 Fc Chimera (Catalog # 10610-B3) inhibits Mouse CD3 epsilon Antibody (Clone # 145-2C11, MAB484) induced proliferation of Mouse T cells. The ED50 for this effect is 0.06-0.54 µg/mL.
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2 μg/lane of Recombinant Rat B7-H3 Fc Chimera Protein (Catalog # 10610-B3) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 66-76 kDa and 132-152 kDa, respectively.
Reconstitution Calculator
Background: B7-H3
B7 homolog 3 (B7-H3), also known as CD276 antigen (CD276), is one member among at least 10 members of the B7 family of immune regulatory proteins within the immunoglobulin superfamily (1-3). The B7 family members all display a conserved extracellular fold but share only about 20-40 % amino acid (aa) sequence identity. Rat B7-H3 consists of an extracellular domain (ECD) containing one V-like and one C-like Ig domain, a helical single-pass type I transmembrane domain, and a cytoplasmic domain. Two isoforms in the ECD of B7-H3 resulting from gene duplication and differential splicing have been identified: one containing four-Ig-like domains (main isoform in humans) and one containing two-Ig-like domains (only isoform in mice) (4, 5). Soluble forms of B7-H3 can result from proteinase cleavage of the isoform with two-Ig-like domains (3). Within the ECD, mature rat B7-H3 shares 92% and 98% aa sequence identity with human and mouse B7-H3, respectively. Human B7-H3 is not expressed on resting B cells, T cells, monocytes or dendritic cells, but is induced on dendritic cells and monocytes by inflammatory cytokines (6, 8). B7-H3 is also overexpressed in numerous cancers including bladder, breast and melanoma (9). Unlike other B7 family members, human B7-H3 does not bind any known members of the CD28 family of immunoreceptors and its receptor has yet to be identified. However, B7-H3 has been shown to bind an unidentified counter-receptor on activated T cells to costimulate the proliferation of CD4+ or CD8+ T cells (10). B7-H3 has also been found to enhance the induction of primary cytotoxic T lymphocytes and stimulate IFN-gamma production (6-8, 10).
- Dong, P. et al. (2018) Front. Oncol. 8:264.
- Castellanos, J. et al. (2017) Am. J. Clin. Exp. Immunol. 6:66.
- Ni, L. and Dong, C. (2017) Mol. Cancer. Ther. 16:1203.
- Shi, T. et al. (2019) Cell Death and Disease. 10: 308.
- Tang, X. et al. (2019) Mol. Ther. Oncolytics. 14:279.
- Chapoval, A.I. et al. (2001) Nat. Immunol. 2:269.
- Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
- Prasad, D.V. et al. (2004) J Immunol. 173:2500.
- Dong, P. et al. (2018) Front Oncol. 8:264.
- Suh, W.K. et al. (2003) Nat Immunol. 4:899.
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