Recombinant R. sanguineus Evasin-1 Fc Chimera Protein, CF

Catalog #: 10016-EV Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10016-EV-050

Recombinant R. sanguineus Evasin-1 Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant R. sanguineus Evasin-1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit CCL3/MIP-1 alpha -induced chemotaxis of BaF3 mouse pro‑B cells transfected with human CCR5. The ED₅₀ for this effect is 0.2-1.2 μg/mL

Source

Human embryonic kidney cell, HEK293-derived r. sanguineus Evasin-1 protein

Human IgG₁ (Met-Asp-Pro100 - Lys330)	IEGR	R. sanguineus Evasin-1 (Glu21-Asn114) Accession # P0C8E7.1
N-terminus		C-terminus

Accession #

P0C8E7.1

N-terminal Sequence
Analysis

Met-Asp-Pro100

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

37 kDa

SDS-PAGE

40-61 kDa, reducing conditions

Product Datasheets

10016-EV

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10016-EV

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Recombinant R. sanguineus Evasin-1 Fc Chimera (Catalog # 10016-EV) inhibits Recombinant Human CCL-3/MIP-1 alpha -induced chemotaxis of BaF3 mouse pro B cells transfected with human CCR5. The ED₅₀ for this effect is 0.2-1.2 μg/mL.

Reconstitution Calculator

Background: Evasin-1

Evasin-1 is a highly selective chemokine-binding protein isolated from tick saliva. The cDNA of tick Evasin-1 encodes a 114 amino acid (aa) precursor, which include a 20 aa signal peptide and the 94 aa mature protein (1). Ticks are blood sucking parasites that secrete a wide variety of immunomodulatory proteins to evade the host immune response. The saliva isolated from Ticks has shown chemokine neutralization activity. A group of proteins contributing to this activity have been identified as chemokine-binding proteins (CHPBs) and named as Evasins (1-3). Evasin-1 shows high affinity binding to a very limited set of three highly homologous CC chemokines: CCL3, CCL4 and CCL18 (1). Since it is a small molecule, Evasin-1 may be therapeutically useful as novel anti-inflammatory agent.

References