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Recombinant Porcine IFN-gamma Protein

Catalog #: 985-PI
16 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
985-PI-050/CF

With Carrier

Catalog # Availability Size / Price Qty
985-PI-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (16)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Porcine IFN-gamma Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured in an anti-viral assay using PK‑15 porcine kidney epithelial cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.015‑0.045 ng/mL.
Source
E. coli-derived porcine IFN-gamma protein
Ser21-Lys166
Accession #
P17803
N-terminal Sequence
Analysis
Ser21
Predicted Molecular Mass
17.3 kDa

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985-PI (with carrier)

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SDS

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985-PI/CF (carrier free)

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

985-PI

Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol,Tween® 80 and TCEP with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

985-PI/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol,Tween® 80 and TCEP.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature porcine IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 72%-79% amino acid sequence identity with bovine, canine, equine, and feline IFN-gamma and 41%-57% with cotton rat, human, mouse, rat, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI ( alpha  subunits) which then interact with IFN-gamma RII ( beta  subunits) to form the functional receptor complex of two alpha  and two beta  subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, anti-proliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

References
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Dijkmans, R. et al. (1990) Nucl. Acids Res. 18:4259.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
Long Name
Interferon gamma
Entrez Gene IDs
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Citations for Recombinant Porcine IFN-gamma Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Effects of IFNgamma and IL4 rich microenvironment on porcine monocyte-derived dendritic cell activation in vitro
    Authors: Z Oreskovic, L Levá, K Chlebová, K Hlavová, R Tesa?ík, J Gebauer, M Faldyna
    Research in Veterinary Science, 2022-02-06;145(0):54-62.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Genetic engineering of porcine endothelial cell lines for evaluation of human-to-pig xenoreactive immune responses
    Authors: P Li, JR Walsh, K Lopez, A Isidan, W Zhang, AM Chen, WC Goggins, NG Higgins, J Liu, RR Brutkiewic, LJ Smith, H Hara, DKC Cooper, B Ekser
    Scientific Reports, 2021-06-23;11(1):13131.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Npro of Classical Swine Fever Virus Suppresses Type III Interferon Production by Inhibiting IRF1 Expression and Its Nuclear Translocation
    Authors: T Cao, X Li, Y Xu, S Zhang, Z Wang, Y Shan, J Sun, W Fang, X Li
    Viruses, 2019-10-31;11(11):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. MicroRNA responses associated with Salmonella enterica serovar typhimurium challenge in peripheral blood: effects of miR-146a and IFN-gamma in regulation of fecal bacteria shedding counts in pig
    Authors: T Huang, X Huang, W Chen, J Yin, B Shi, F Wang, W Feng, M Yao
    BMC Vet. Res., 2019-06-11;15(1):195.
    Species: Porcine
    Sample Types: In Vivo
    Applications: In Vivo
  5. In vitro characterization of PRRSV isolates with different in vivo virulence using monocyte-derived macrophages
    Authors: G Ogno, CA Sautter, E Canelli, O García-Nic, T Stadejek, P Martelli, P Borghetti, A Summerfiel
    Vet. Microbiol., 2019-03-11;231(0):139-146.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Analysis of stage-specific expression of the toll-like receptor family in the porcine endometrium throughout the estrous cycle and pregnancy
    Authors: I Yoo, J Han, S Lee, W Jung, JH Kim, YW Kim, HJ Kim, M Hong, H Ka
    Theriogenology, 2018-11-11;125(0):173-183.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: Bioassay
  7. Infection of monocytes with European porcine reproductive and respiratory syndrome virus (PRRSV-1) strain Lena is significantly enhanced by dexamethasone and IL-10
    Authors: H Singleton, SP Graham, JP Frossard, KB Bodman-Smi, F Steinbach
    Virology, 2018-03-02;0(0):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  8. A Subretinal Cell Delivery Method via Suprachoroidal Access in Minipigs: Safety and Surgical Outcomes
    Authors: MD de Smet, JL Lynch, NS Dejneka, M Keane, IJ Khan
    Invest. Ophthalmol. Vis. Sci., 2018-01-01;59(1):311-320.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Experimental Porcine Toxoplasma gondii Infection as a Representative Model for Human Toxoplasmosis
    Authors: J Nau, SK Eller, J Wenning, KH Spekker-Bo, H Schroten, C Schwerk, A Hotop, U Gro beta, W Däubener
    Mediators Inflamm., 2017-08-13;2017(0):3260289.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Characterization of interferon ? and ? receptor IFNAR1 and IFNAR2 expression and regulation in the uterine endometrium during the estrous cycle and pregnancy in pigs
    Authors: Hakhyun Ka
    Theriogenology, 2016-09-22;88(0):166-173.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: Bioassay
  11. Genome-wide analysis of antiviral signature genes in porcine macrophages at different activation statuses.
    Authors: Sang Y, Brichalli W, Rowland R, Blecha F
    PLoS ONE, 2014-02-05;9(2):e87613.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Regulation of porcine plasmacytoid dendritic cells by cytokines.
    Authors: Lannes N, Summerfield A
    PLoS ONE, 2013-04-08;8(4):e60893.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  13. IFN-alpha treatment enhances porcine Arterivirus infection of monocytes via upregulation of the porcine Arterivirus receptor sialoadhesin.
    Authors: Delputte PL, Van Breedam W, Barbe F, Van Reeth K, Nauwynck HJ
    J. Interferon Cytokine Res., 2007-09-01;27(9):757-66.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Although pig allogeneic mesenchymal stem cells are not immunogenic in vitro, intracardiac injection elicits an immune response in vivo.
    Authors: Poncelet AJ, Vercruysse J, Saliez A, Gianello P
    Transplantation, 2007-03-27;83(6):783-90.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Inhibition of microglia multinucleated giant cell formation and induction of differentiation by GM-CSF using a porcine in vitro model.
    Authors: Tambuyzer BR, Nouwen EJ
    Cytokine, 2005-08-21;31(4):270-9.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  16. The NS1 gene of H5N1 influenza viruses circumvents the host anti-viral cytokine responses.
    Authors: Seo SH, Hoffmann E, Webster RG
    Virus Res., 2004-07-01;103(1):107-13.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay

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