Recombinant Mouse TREML1/TLT-1 Protein, CF Summary
Product Specifications
When 5 x 106 cells per well are added to rmTLT-1 coated plates (2 μg/mL, 100 μL/well), approximately 50%-70% will adhere after 1 hour at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Gly18-Cys178 with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2424-TL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: TREML1/TLT-1
Triggering receptor expressed on myeloid cells-like protein 1 (TREML1)/TREM-like transcript-1 (TLT-1) is a 40 kDa type I transmembrane receptor and member of the TREM family of proteins (1). Mouse TREML1/TLT-1 is synthesized as a 317 amino acid (aa) precursor that contains a 20 aa signal sequence, a 155 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 121 aa cytoplasmic domain (SwissProt #: Q8K558). The ECD contains an Ig-like V-type domain (aa 21 - 122), and the cytoplasmic region contains a proline-rich region (aa 245 - 275) and an immunoreceptor tyrosine-based inhibition motif (ITIM) characterized by the residues S/I/V/LXYXXV/L (1). A splicing variant produces a second isoform that has a six aa substitution for aa 173 in isoform 1. Mature mouse TREML1/TLT-1 shares 65% aa sequence identity with mature human TREML1/TLT-1. TREML1/TLT-1 is expressed exclusively in megakaryocytes and platelets where it colocalizes with CD62P in alpha -granules in resting platelets and on the cell surface of platelets activated by thrombin (1 - 3). The receptor’s expression is upregulated dramatically upon platelet activation (2). Antibodies to single-chain Fv fragments specific for TLT-1 inhibited thrombin-mediated human platelet aggregation suggesting its role in the regulation of aggregation (5). In addition, soluble fragments including the ECD can be released into serum by proteolysis (3). The phosphorylated TREML1/TLT-1 is able to interact with both SHP-1 and SHP-2 through ITIM (1, 4). SHP-2 interaction enhances Fc epsilon RI-mediated calcium signaling in rat basophilic leukemia cells (1). It is hypothesized that TREML1/TLT-1 plays a role in the innate and adaptive immune responses.
- Barrow, A.D. et al. (2004) J. Immunol. 172:5838.
- Washington, A.V. et al. (2004) Blood 104:1042.
- Gattis, J.L. et al. (2006) J. Biol. Chem. 281:13396.
- Washington, A.V. et al. (2002) Blood 100:3822.
- Giomarelli B. et al. (2007) Thromb. Haemost. 97:955.
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