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Recombinant Mouse TRAIL/TNFSF10 Protein

Catalog #: 1121-TL
7 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
1121-TL-010/CF

With Carrier

Catalog # Availability Size / Price Qty
1121-TL-010
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (7)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse TRAIL/TNFSF10 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 0.2-1.2 ng/mL.
Source
E. coli-derived mouse TRAIL/TNFSF10 protein
Pro118-Asn291
Accession #
P50592
N-terminal Sequence
Analysis
Pro118
Predicted Molecular Mass
20 kda

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1121-TL (with carrier)

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1121-TL/CF (carrier free)

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COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1121-TL

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, Ammonium Sulfate and ZnSO4 with Trehalose and with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS. 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1121-TL/CF

Formulation Supplied as a 0.2 μm filtered solution in Tris, Ammonium Sulfate and ZnSO4.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRAIL/TNFSF10

TNF-related apoptosis-inducing ligand (TRAIL), also called apoptosis 2 ligand (Apo2L) for its similarity in sequence, structure, and function to Fas Ligand/Apo1L, is a 33-35 kDa type II transmembrane glycoprotein of the tumor necrosis factor superfamily, designated TNFSF10 (1‑3). Mouse TRAIL cDNA encodes a 17 amino acid (aa) N-terminal intracellular domain, a 20 aa transmembrane domain and a 253 aa extracellular domain. Like most TNF family members, TRAIL is bioactive as a homotrimer (1). Unlike other TNF family members, a zinc ion complexed by human Cys 230 (mouse Cys 240) of each of the three monomers is critical for structural stability (4, 5). Either transmembrane or cysteine protease-released soluble sTRAIL induce apoptosis of many transformed cell lines, but rarely of normal cells (3, 6). Accordingly, TRAIL is suggested to have a role in tumor surveillance (1). Mice with genetically disrupted TRAIL have defective thymocyte apoptosis, creating faulty negative selection and some increased susceptibility to induced autoimmune diseases (7). In humans, TRAIL controls apoptosis of erythrocyte precursors and sTRAIL is inversely correlated with hemoglobin (1, 8). TRAIL transcripts are constitutively expressed in a variety of human (and presumably mouse) tissues and mononuclear cells (2, 3). Only one of two receptors that transduce apoptotic signals in humans is found in the mouse (TRAIL R2/DR5 but not TRAIL R1/DR4) (1). Mice express TRAIL receptors DcTRAIL R1/TNFRSF23 and DcTRAIL R2/TNFRSF22. These receptors lack death domains, but differ in structure from human regulatory receptors TRAIL R3 and TRAIL R4 (9). Osteoprotegerin has been identified in humans as a TRAIL receptor, but binding in mouse has not yet been demonstrated (1, 10). Mouse TRAIL shows 85% aa identity with rat TRAIL and 70% aa identity with human, bovine, and porcine TRAIL within the TNF homology domain (aa 118-291).

References
  1. Zauli, G. and P. Secchiero (2006) Cytokine Growth Factor Rev. 17:245.
  2. Wiley, S.R. et al. (1995) Immunity 3:673.
  3. Pitti, R.M. et al. (1996) J. Biol. Chem. 271:12687.
  4. Bodmer, J.L. et al. (2000) J. Biol. Chem. 275:20632.
  5. Hymowitz, S.G. et al. (2000) Biochemistry 39:633.
  6. Sedger, L. M. et al. (2002) Eur. J. Immunol. 32:2246.
  7. Lamhamedi-Cherradi, S.E. et al. (2003) Nat. Immunol. 4:255.
  8. Choi, J.W. (2005) Ann. Hematol. 84:728.
  9. Schneider, P. et al. (2003) J. Biol. Chem. 278:5444.
  10. Emery, J. et al. (1998) J. Biol. Chem. 273:14363.
Long Name
TNF-related Apoptosis-inducing Ligand
Entrez Gene IDs
8743 (Human); 22035 (Mouse); 246775 (Rat)
Alternate Names
Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAIL; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10

Citations for Recombinant Mouse TRAIL/TNFSF10 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Aldehyde Dehydrogenase 1 (ALDH1) Promotes the Toxicity of TRAIL in Non-Small Cell Lung Cancer Cells via Post-Transcriptional Regulation of MEK-1 Expression
    Authors: S Tian, DH Liu, D Wang, F Ren, P Xia
    Cell. Physiol. Biochem., 2018-11-16;51(1):217-227.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Two-signal requirement for growth-promoting function of Yap in hepatocytes.
    Authors: Su, Tian, Bondar, Tanya, Zhou, Xu, Zhang, Cuiling, He, Hang, Medzhitov, Ruslan
    Elife, 2015-02-10;4(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) signaling and cell death in the immature central nervous system after hypoxia-ischemia and inflammation.
    Authors: Kichev A, Rousset C, Baburamani A, Levison S, Wood T, Gressens P, Thornton C, Hagberg H
    J Biol Chem, 2014-02-07;289(13):9430-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Tumor necrosis factor-related apoptosis-inducing ligand translates neonatal respiratory infection into chronic lung disease.
    Authors: Starkey M, Nguyen D, Essilfie A, Kim R, Hatchwell L, Collison A, Yagita H, Foster P, Horvat J, Mattes J, Hansbro P
    Mucosal Immunol, 2013-09-18;7(3):478-88.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012-06-04;109(25):9971-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. TNF superfamily member, TL1A, is a potential mucosal vaccine adjuvant.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Katayama K, Yoshida T, Yamashita K, Yoshikawa T, Hiroi T, Itoh N, Kawai Y, Mayumi T, Kamada H, Tsunoda S, Tsutsumi Y
    Biochem. Biophys. Res. Commun., 2009-05-04;384(3):296-300.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. Doxorubicin enhances TRAIL-induced cell death via ceramide-enriched membrane platforms.
    Authors: Dumitru CA, Carpinteiro A, Trarbach T, Hengge UR, Gulbins E
    Apoptosis, 2007-08-01;12(8):1533-41.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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