Recombinant Mouse TLR9 Fc Chimera Protein, CF

Catalog #: 7960-TR
1 Citation Datasheet / COA / SDS

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7960-TR-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse TLR9 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to bind biotinylated CpG oligodeoxynucleotide, type B. The 50% maximum binding is achieved at 30‑150 ng/mL of Recombinant Mouse TLR9 Fc Chimera.
Optimal dilutions should be determined by each laboratory for each application.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse TLR9 protein

Mouse TLR9 (Leu26-Asp818) Accession # AAK29625	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

AAK29625

N-terminal Sequence
Analysis

Leu26

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

115.8 kDa (monomer)

SDS-PAGE

140-170 kDa, reducing conditions

Product Datasheets

7960-TR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7960-TR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: TLR9

TLR9 (Toll‑like receptor 9), designated CD289, is a member of the TLR family of innate immune receptors that is mainly expressed by colonic epithelium, CD123⁺ plasmacytoid predendritic cells (pDC), and splenic transitional B cells (1‑9). TLR9 responds to unmethylated DNA CpG motifs that occur mainly in bacteria and viruses (1, 2). Mouse TLR9 cDNA encodes a 1032 amino acid (aa) type I transmembrane glycoprotein with a 793 aa extracellular domain (ECD) that contains 26 leucine‑rich repeats (LRRs, aa 26‑818), and a 193 aa cytoplasmic domain with a TIR sequence that dimerizes with signaling adaptors such as MyD88 (1). The mouse TLR9 ECD shares 87% aa sequence identity with rat and 71‑74% with human, feline, canine, equine, porcine, bovine and ovine TLR9. Predicted splice forms vary at the N‑terminus by initiating either upstream or downstream of the standard site. The full-length 150 kDa form, which is ligand‑binding but nonsignaling, is found in the endoplasmic reticulum. It undergoes accessory protein-mediated translocation either to the cell membrane or to lysosomes (1‑3). TLR9 is cleaved to remove LRR1‑14, producing an 80 kDa signaling fragment within acidic endolysosomes where it encounters microbial CpG DNA rather than self-DNA (2, 10, 11). However, immune complexes of self‑DNA with lupus erythematosus anti‑DNA antibodies can induce TLR9 activation and IFN‑ alpha production in pDC (4). A soluble form also found in endosomes includes all 26 LRRs and negatively regulates active TLR9 (12). Activation of TLR9 contributes to splenocyte proliferation, pDC maturation, macrophage inflammatory cytokine production, Th1 inflammatory responses, NK cell activation and recruitment, B cell surface MHC class II up‑regulation and immunoglobulin production, and generation and maintenance of memory B cells (1, 5‑9).

References

Hemmi, H. et al. (2000) Nature 408:740.
Barton, G.M. et al. (2006) Nat. Immunol. 7:49.
Latz, E. et al. (2004) Nat. Immunol. 5:190.
Barrat, F.J. et al. (2005) J. Exp. Med. 202:1131.
Krieg, A.M. et al. (1995) Nature 374:546.
Aranburu, A. et al. (2010) J. Immunol. 185:7293.
Guerrier, T. et al. (2012) J. Autoimmun. 39:173.
Guillerey, C. et al. (2012) Blood 120:90.
Cunningham-Rundles, C. et al. (2006) J. Immunol. 176:1978.
Ewald, S.E. et al. (2008) Nature 456:658.
Park, B. et al. (2008) Nat. Immunol. 9:1407.
Chockalingam, A. et al. (2011) Eur. J. Immunol. 41:2176.

Long Name

Toll-like Receptor 9

Entrez Gene IDs

54106 (Human); 81897 (Mouse); 338457 (Rat)

Alternate Names

CD289 antigen; CD289; TLR9; toll-like receptor 9

Related Research Areas

Citation for Recombinant Mouse TLR9 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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