Recombinant Mouse PLTP Protein Summary
Product Specifications
Glu18-Ala493, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4918-PL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
4918-PL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: PLTP
Phospholipid transfer protein (PLTP; also known as lipid transfer protein II) is an 80 kDa, secreted glycoprotein, that belongs to the BPI/LBP/Plunc superfamily of molecules (1). It is synthesized as a precursor with a 17 amino acid (aa) signal sequence and a 476 aa mature chain. The mature chain contains two BPI/LBP/CETP domains (aa 21 - 239 and 263 - 463) and seven potential sites for N-linked glycosylation. Mature mouse PLTP shares 83% aa sequence identity with mature human PLTP (3). PLTP is expressed by a wide variety of tissues and is secreted by macrophages. Northern blot analysis shows the order of PLTP mRNA expression as follows: lung > adipose tissue, placenta, testis > brain > muscle, heart, liver (2). PLTP is a multifunctional, extracellular lipid transfer protein that plays a major role in lipoprotein metabolism and atherosclerosis (4 - 6). In addition to phospholipids, PLTP transfers several amphipatic compounds, including unesterified cholesterol, lipopolysaccharides, diacylglycerides, and alpha -tocopherol, the main isomer of vitamin E (4). According to several studies, PLTP not only affects plasma lipid and lipoprotein profiles, but also modulates cell physiology (4, 7 - 11). In mice, systemic PLTP deficiency proves to be atheroprotective, whereas PLTP overexpression is proatherogenic (4, 6). However, macrophage PLTP is shown to be atheroprotective (4, 6).
- Jauhiainen, M. and C. Ehnholm (2005) Methods 36:97.
- Jiang, X.-C. and C. Bruce (1995) J. Biol. Chem. 270:17133.
- Albers, J.J. et al. (1995) Biochim. Biophys. Acta. 1258:27.
- Ogier, N. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:2407.
- Huuskonen, J. and C. Ehnholm (2000) Curr. Opin. Lipidol. 11:285.
- Valenta, D.T. et al. (2008) J. Lipid Res. 49:24.
- Jiang, X.-C. et al. (1998) J. Biol. Chem. 273:15714.
- Jiang, X.-C. et al. (2005) J. Biol. Chem. 280:18336.
- Desrumaux, C. et al. (2005) FASEB J. 19:296.
- Drouineaud, V. et al. (2006) FASEB J. 20:794.
- Klein, A. et al. (2006) Arterioscler. Thromb. Vasc. Biol. 26:2160.
Citation for Recombinant Mouse PLTP Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Cathepsin G degradation of phospholipid transfer protein (PLTP) augments pulmonary inflammation.
Authors: Brehm A, Geraghty P, Campos M, Garcia-Arcos I, Dabo A, Gaffney A, Eden E, Jiang X, D'Armiento J, Foronjy R
FASEB J, 2014-02-14;28(5):2318-31.
Species: Human
Sample Types: BALF
Applications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Mouse PLTP Protein
There are currently no reviews for this product. Be the first to review Recombinant Mouse PLTP Protein and earn rewards!
Have you used Recombinant Mouse PLTP Protein?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
