Recombinant Mouse PIR-B Protein, CF Summary
Product Specifications
Gly24-Gly635, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2754-PB
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with trehalose |
| Reconstitution | Reconstitute at 250 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: PIR-B
Paired immunoglobulin‑like receptor B (PIR‑B) is a transmembrane protein that acts as a negative regulator of immune responses. It is the rodent homolog of human LILRB1‑5 family proteins. It is most closely related to LILRB3 which is also known as ILT5, LIR‑3, and CD85a. Like LILRB family members, PIR‑B contains cytoplasmic immunoreceptor tyrosine‑based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP‑1. PIR‑A and other LILRA family members instead contain cytoplasmic ITAMs which augment immune responses (1). Mature mouse PIR‑B is a 120 kDa glycoprotein that consists of a 618 amino acid (aa) extracellular domain (ECD) with six Ig‑like domains, a 21 aa transmembrane segment, and a 178 aa cytoplasmic domain with four ITIMs (2). The 85‑95 kDa human ILT5 contains four Ig‑like domains. Within common regions of their ECDs, mouse PIR‑B shares 73% and 55% aa sequence identity with rat PIR‑B and human ILT5, respectively. PIR‑B is expressed on dendritic cells, monocytes, macrophages, B cells, mast cells, neutrophils, eosinophils, basophils, osteoclasts, and cerebellar granule neurons (3‑10). Triggering of PIR‑B inhibits the activation of macrophages, mast cells, neutrophils, basophils, and B cells (5‑7, 9). PIR‑B also inhibits the differentiation of osteoclasts and pro‑inflammatory M1 macrophages (4, 11). PIR‑B interacts in cis and in trans with MHC I molecules to limit autoreactive humoral and cellular immune responses (12‑14). It suppresses anti‑bacterial inflammatory responses by blocking TLR9‑dependent B cell activation (14). In the CNS, PIR‑B functions as a receptor for the myelin proteins Nogo, MAG, and OMgp and mediates their inhibitory action on neurite outgrowth and axon regeneration (10). Upon binding to MAG, PIR‑B associates with TrkB and NGF R/p75 in cerebellar granule neurons (15).
- Anderson, K.J. and R.L. Allen (2009) Immunology 127:8.
- Hayami, K. et al. (1997) J. Biol. Chem. 272:7320.
- Mitsuhashi, Y. et al. (2012) Blood 120:3256.
- Mori, Y. et al. (2008) J. Immunol. 181:4742.
- Pereira, S. et al. (2004) J. Immunol. 173:5757.
- Uehara, T. et al. (2001) J. Clin. Invest. 108:1041.
- Ujike, A. et al. (2002) Nat. Immunol. 3:542.
- Tedla, N. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1174.
- Sloane, D.E. et al. (2004) Blood 104:2832.
- Atwal, J.K. et al. (2008) Science 322:967.
- Ma, G. et al. (2011) Immunity 34:385.
- Nakamura, A. et al. (2004) Nat. Immunol. 5:623.
- Endo, S. et al. (2008) Proc. Natl. Acad. Sci. USA 38:14515.
- Kubo, T. et al. (2009) J. Exp. Med. 206:1971.
- Fujita, Y. et al. (2011) Cell Death Dis. 2:e198.
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Reason for Rating: Could not see cross reactivity of human HLA-G to murine PIR-B. Did see binding to human ILTs
Immobilize HLA-G and flow PIR-B
