Recombinant Mouse PDGF-AB Protein, CF

Catalog #: 10839-AB Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10839-AB-010
10839-AB-050

Recombinant Mouse PDGF-AB Protein Bioactivity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse PDGF-AB Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 15.0-75.0 ng/mL.

Source

E. coli-derived mouse PDGF-AB protein

Mouse PDGF-A
(Ser87-Thr211)
Accession # P20033.2

Mouse PDGF-B (Ser82-Thr190) Accession # P31240.1
N-terminus	C-terminus

Accession #

P20033.2 (A chain) & P31240.1 (B chain)

N-terminal Sequence
Analysis

Ser87 (A chain) & Ser82 (B chain)

Structure / Form

Disulfide-linked heterodimer

Predicted Molecular Mass

14 kDa (A chain) & 12 kDa ( beta chain)

SDS-PAGE

17.5 kDa (A chain) & 12.5 kDa (B chain), under reducing conditions.

Product Datasheets

10839-AB

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10839-AB

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse PDGF-AB induces NR6R-3T3 mouse fibroblast cell proliferation. The ED₅₀ for this effect is 15.0-75.0 ng/mL.

SDS-PAGE

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2 µg/lane of Recombinant Mouse PDGF-AB (Catalog # 10839-AB) was resolved by SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 17.5 kDa (A chain) & 12.5 kDa (B chain) and 29 kDa (heterodimer), respectively.

Reconstitution Calculator

Background: PDGF-AB

Platelet-Derived Growth Factors (PDGFs) are a family of four cystine-knot-type growth factors (PDGF-A, -B, -C and -D) which control the growth of connective tissue cells such as fibroblasts and smooth muscle cells (1, 2). They comprise of four homodimers (PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD) and one heterodimer (PDGF-AB). All four PDGF genes contain a signal sequence, a propeptide and a mature form (2, 3). Mature mouse PDGFA is 95% and 98% identical to human and rat PDGFA respectively whereas mature mouse PDGFB is 89% and 98% homologous to human and rat PDGFB respectively. In general, PDGFs are expressed by a number of different cells and tissues including endothelial cells, epithelial cells, hematopoietic cells, connective tissue, nervous tissue, brain, muscle, kidney and liver. PDGF expression is highly regulated with PDGF levels increasing following injury and/or disease. An analysis of PDGF purified from human platelets suggest that approximately 70% of sera-derived PDGF is PDGF-AB (4). PDGF isoforms exert their cellular effect by binding to the structurally similar receptor tyrosine kinases PDGF receptor-alpha and PDGF receptor-beta. PDGF-AB binding induces receptor dimerization resulting in alpha alpha receptor homodimers and alpha beta receptor heterodimers. Binding of PDGF receptors has been reported to result in stimulation of mitogenicity and chemotaxis of fibroblasts, stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, stimulation of collagen, fibronectin, proteoglycan, and hyaluronic acid synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production (5).

References

Kohler, N. Lipton, A. (1974) Exp Cell Res. 87:297.
Ross, R. et al. (1974) Proc Natl Acad Sci U.S.A. 71:1207.
Frediksson, L. et al. (2004) Cytokine Growth Factor Rev. 15:197.
Chen, P-H. et al. (2013) BiochimBiophysActa 1834:2176.
Heldin, C-H. Eriksson, U. and Ostman, A. (2002) Arch. Biochem. Biophysic. 398:284.