Recombinant Mouse Norrin Protein Summary
Product Specifications
Lys25-Ser131, with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3497-NR
| Formulation | Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
3497-NR/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in HCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Norrin
Norrin (also called Norrie Disease Protein or NDP) is a secreted regulatory protein that remains tightly associated with the extracellular matrix (1). At least 70 mutations of human Norrin have been identified in Norrie disease (X-linked retinal dysplasia) or in a minority of X-linked familial exudative vitreoretinopathy (FEVR). Both are disorders of retinal vascularization that cause blindness (2, 3). Norrin consists of disulfide-linked homodimers that oligomerize further via disulfide bridges to form higher order oligomers containing up to ten 12 kDa units. The cysteine-rich C-terminal domain of Norrin shows homology to von Willebrand factor, several extracellular mucin proteins, and members of the TGF-beta family. Molecular modeling studies predict that Norrin assumes the cysteine-knot structure typical of the TGF-beta family (4). Although Norrin is not related to Wnt family proteins, it functions like a Wnt protein in that it binds with high affinity to the receptor Frizzled-4, requires LDL receptor-related protein (LRP) as a co-receptor, and induces activation of the canonical Wnt signaling pathway (5). Norrin and Frizzled-4 are widely expressed, but at relatively low levels, in tissues displaying vascular phenotypes. Genetic disruption of either Norrin or Frizzled-4 results in blindness and progressive deafness due to vascular abnormalties (5-7). Female Norrin-/- mice are infertile due to abnormalities in decidual vasculature (8). Mature mouse Norrin shares 97-98% aa identity with human, canine and bovine Norrin.
- Perez-Vilar, J. and R.L. Hill (1997) J. Biol. Chem. 272:33410.
- Berger, W. et al. (1992) Nat. Genet. 1:199.
- Berger, W. and H.H. Ropers, 2001, in “The Metabolic and Molecular Bases of Inherited Diseases”, C.R. Scriver, et al., eds., pp. 5977.
- Meitinger, T. et al. (1993) Nat. Genet. 5:376.
- Xu, Q. et al. (2004) Cell 116:883.
- Hartzer, M.K. et al. (1999) Brain Res. Bull. 49:355.
- Wang, Y. et al. (2001) J. Neurosci. 21:4761.
- Luhmann, U.F.O. et al. (2005) Genesis 42:253.
Citations for Recombinant Mouse Norrin Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Dysregulation of Wnt/?-catenin signaling contributes to intestinal inflammation through regulation of group 3 innate lymphoid cells
Authors: Hao, J;Liu, C;Gu, Z;Yang, X;Lan, X;Guo, X;
Nature communications
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The tyrosine Y250(2.39) in Frizzled 4 defines a conserved motif important for structural integrity of the receptor and recruitment of Disheveled
Authors: K Strakova, P Matricon, C Yokota, E Arthofer, O Bernatik, D Rodriguez, E Arenas, J Carlsson, V Bryja, G Schulte
Cell. Signal., 2017-06-29;38(0):85-96.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Wnt signaling in form deprivation myopia of the mice retina.
Authors: Ma M, Zhang Z, Du E, Zheng W, Gu Q, Xu X, Ke B
PLoS ONE, 2014-04-22;9(4):e91086.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
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