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Recombinant Mouse LRP-6 N-Terminal Fragment Fc Protein, CF

Catalog #: 9950-LR Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9950-LR-050
Recombinant Mouse LRP-6 N-Terminal Fragment Fc Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse LRP-6 N-Terminal Fragment Fc Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. In a Goat Anti-Mouse IgG Fc Antibody (Catalog # G-202-C) coated plate, Recombinant Mouse LRP‑6 N-Terminal Fragment Fc Chimera binds Recombinant Mouse Dkk-1 (Catalog # 5897-DK) with an ED50 of 15-120 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse LRP-6 protein
Mouse LRP-6
(Ala20-Ile628)
Accession # NP_032540		IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
NP_032540
N-terminal Sequence
Analysis
Ala20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
96 kDa
SDS-PAGE
100-120 kDa, reducing conditions

Product Datasheets

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9950-LR

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9950-LR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Mouse LRP-6 N-Terminal Fragment Fc Protein Binding Activity View Larger

In a Goat Anti-Mouse IgG Fc Antibody (Catalog # G-202-C) coated plate, Recombinant Mouse LRP-6 N-Terminal Fragment Fc Chimera (Catalog # 9950-LR) binds Recombinant Mouse Dkk-1 (Catalog # 5897-DK) with an ED50 of 15-120 ng/mL.

SDS-PAGE Recombinant Mouse LRP-6 N-Terminal Fragment Fc Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse LRP‑6 N-Terminal Fragment Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 100-120 kDa and 200-220 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRP-6

The low-density lipoprotein (LDL) receptor-related protein 6 (LRP-6), along with LRP-5, constitute a distinct subgroup of the LDL receptor family (1). Both LRP-5 and LRP-6 are type I transmembrane proteins that function as Wnt co-receptors with Frizzled proteins and mediate the down-regulation of GSK-3 activity and the initiation of the canonical Wnt/beta-catenin signaling cascade (1, 2). Mouse LRP-6 consists of a 19 amino acid (aa) signal sequence, a 1351 aa extracellular domain (ECD), a 23 aa transmembrane domain, and a 20 aa cytoplasmic domain (3). The LRP-6 ECD is composed of four distinct YWTD beta -propeller (BP) motifs followed by three LDLR type A repeats (4). The first two LRP-6 BP motifs bind Wnt-1, -2, -2b, -6, -8a, -9a, -9b, and -10b, while the third and fourth BP motifs bind Wnt-3a (5-10). This product includes the first and second BP motifs of mouse LRP-6 (aa 20-628) that share 98% aa sequence identity with the first two BP motifs of rat and human LRP-6.  Dkk-1, a known antagonist of Wnt signaling, interacts with LRP-6 as a bipartite inhibitor, binding the first and third BP motifs of LRP-6 simultaneously (8, 11). Mice lacking LRP-6 die at birth with a truncation of the axial skeleton, limb defects, microphthalmia, and malformation of the urogenital system (2). Additionally, LRP-6 may be essential for osteoblast differentiation during bone remodeling and, along with LRP-5, is required for postnatal bone development (12, 13).

References
  1. Joiner, D.M. et al. (2013) Trends Endocrinol. Metab. 24:31.
  2. Pinson, K.I. et al. (2000) Nature 407:535.
  3. Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248:879.
  4. He, X. et al. (2004) Development 131:1663.
  5. Ai, M. et al. (2005) Mol. Cell. Biol. 25:4946.
  6. Gong, Y. et al. (2010) PLoS One 5:e12682.
  7. Itasaki, N. et al. (2003) Development 130:4295.
  8. Mao, B. et al. (2001) Nature 411:321.
  9. Zhang, Y. et al. (2004) Mol. Cell. Biol. 24:4677.
  10. Bourhis, E. et al. (2010) J. Biol. Chem. 285:9172.
  11. Ahn, V.E. et al. (2011) Dev. Cell 21:862.
  12. Li, C. et al. (2013) J. Bone Miner. Res. 28:2094.
  13. Riddle, R.C. et al. (2013) PLoS One 8:e63323.
Long Name
LDL Receptor-related Protein 6
Entrez Gene IDs
4040 (Human); 16974 (Mouse)
Alternate Names
ADCAD2; FLJ90062; FLJ90421; low density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP6; LRP-6

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