Recombinant Mouse Lgr4/GPR48 Fc Chimera Protein, CF

Catalog #: 8077-GP Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8077-GP-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Lgr4/GPR48 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse R‑Spondin 3 (Catalog # 4120-RS) is coated at 0.5 μg/mL, Recombinant Mouse Lgr4/GPR48 Fc Chimera binds with an apparent K_d <2nM.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Lgr4/GPR48 protein

Mouse Lgr4/GPR48 (Gly20-Thr544) Accession # A2ARI4	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

A2ARI4

N-terminal Sequence
Analysis

Gly20 & Ala25

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

84.6 kDa (monomer)

SDS-PAGE

100-130 kDa, reducing conditions

Product Datasheets

8077-GP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8077-GP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 400 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Lgr4/GPR48

Lgr4 (leucine-rich repeat G-protein coupled receptor 4), also called GPR48, is a seven-transmembrane glycoprotein receptor in the Lgr family of cell surface receptors (1, 2). While this family includes receptors for hormones such as LH, FSH, TSH, and HCG, the subfamily comprising Lgr4, Lgr5, and Lgr6 are G-protein independent mediators of the potentiating effect of R-Spondins on Wnt signaling (1-6). Lgr4 binds and forms complexes with R-Spondins, Frizzled Wnt receptors and LRP Wnt
co-receptors (5). It acts at least in part by enhancing Wnt-dependent LRP phosphorylation, internalization of LRPs, and accumulation of beta -catenin (3, 4). Mouse Lgr4 cDNA encodes 951 amino acids (aa), including a long N-terminal extracellular domain (ECD, aa 25-544) with 16-17 LRR domains that mediate ligand interaction (1). The LRR-containing ECD of mouse Lgr4 shares 93%, 97% and 90% aa sequence identity with human, rat and bovine Lgr4, respectively, and 52-53% aa identity with mouse Lgr5 and Lgr6. Rodent Lgr4 is widely expressed in both embryo and adult, with mRNA found in tissues including liver, kidney, adrenals, bone/cartilage, and heart (2, 7-9). Expression of Lgr4 mRNA in adult humans is highest in pancreas, followed by liver, heart, muscle, brain, and placenta (1). Lgr4 deletion in the mouse affects development in areas of expression, for example, inhibiting fetal liver definitive erythropoiesis, and arresting germ cell meiosis, thus blocking spermatogenesis (9, 10). Deletion of Lgr4 specifically from stem and progenitor cells in intestinal crypts induces loss of crypts due to insufficient Wnt signaling. LGR4^-/- mice show hyperactivity of innate immune responses, and are also susceptible to more severe inflammatory bowel disease induction (5, 6, 11, 12). Lgr4 may be over-expressed in carcinomas and may promote invasiveness and metastasis by down‑regulating p27^Kip1 expression (13).

References