Recombinant Mouse Insulin R/CD220 Protein, CF

Catalog #: 7544-MR
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7544-MR-050

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Citations (2)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Insulin R/CD220 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When 15 ng/mL of biotinylated recombinant human Insulin is added to serially diluted Recombinant Mouse Insulin R/CD220, the concentration of Recombinant Mouse Insulin R/CD220 that produces 50% of the optimal binding response is 0.3-1.2 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Insulin R/CD220 protein
His28-Ser748 ( alpha subunit) & Ser753-Lys946, with a C-terminal 6-His tag ( beta subunit)

Accession #

P15208

N-terminal Sequence
Analysis

His28 ( alpha subunit) & Ser753 ( beta subunit)

Predicted Molecular Mass

82.6 kDa ( alpha subunit) & 22.5 kDa ( beta subunit)

SDS-PAGE

120-150 & 38-41 kDa, reducing conditions

Product Datasheets

7544-MR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7544-MR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Insulin R/CD220

The Insulin Receptor (gene name INSR, designated CD220) is a type I transmembrane glycoprotein in the Insulin/IGF Receptor family of receptor tyrosine kinases that share structural similarity and overlapping intracellular signaling events (1-3). The 1382 amino acid (aa) mouse Insulin R preproprotein is processed by proteolysis to remove the signal peptide and produce an extracellular alpha portion (aa 28-748), and an extracellular/transmembrane/cytoplasmic beta subunit (aa 753-1372) (4). The extracellular domain (ECD) contains two homologous globular domains separated by a cysteine-rich domain and followed by three fibronectin type III domains. The intracellular region contains insulin-receptor substrate (IRS) docking sites, the kinase domain, and a phosphotyrosine-containing linker region. The Mouse Insulin R ECD shares 96%, 99%, 94% and 94% aa sequence identity with human, rat, equine and canine Insulin R, respectively. Insulin R may homodimerize, or heterodimerize with the IGF-I receptor (1, 3, 4). All receptor combinations bind insulin, IGF-I or IGF-II, but with differing affinities (2-5). This system allows fine tuning of signaling pathways according to the concentrations of insulin, IGF-I and IGF-II, and expression of receptor subunits on the cell surface (2, 3). Insulin R signaling regulates glucose uptake and metabolism, but also contributes to cell growth, differentiation and apoptosis (2, 3, 5, 6). Consistent with mice deleted for Insulin R, mutations in the human Insulin R gene have been linked severe insulin resistance (type A and Rabson-Mendenhall syndrome) that may include type II diabetes mellitus and, rarely, leprechaunism (Donohue syndrome) that also includes growth delays and endocrine system abnormalities (1, 7). The R&D Systems mouse Insulin R consists of the entire ECD and is a pre-receptor that does not contain bound insulin.

References

Nakae, J. et al. (2001) Endoc. Rev. 22:818.
Sciacca, L. et al. (2003) Endocrinology 144:2650.
Belfiore, A. et al. (2009) Endocrine Rev. 30:586.
Lawrence, M.C. et al. (2007) Curr. Opin. Struct. Biol. 17:699.
Sacco, A. et al. (2009) Endocrinology 150:3594.
Kitamura, T. et al. (2004) J. Clin. Invest. 113:209.
Musso, C. et al. (2004) Medicine (Baltimore) 83:209.

Long Name

Insulin Receptor

Entrez Gene IDs

3643 (Human); 16337 (Mouse)

Alternate Names

CD 220; CD220 antigen; CD220; EC 2.7.10; EC 2.7.10.1; HHF5; INSR; Insulin R; insulin receptor; InsulinR; IR

Related Research Areas

Citations for Recombinant Mouse Insulin R/CD220 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Identification of lamprey variable lymphocyte receptors that target the brain vasculature
Authors: JM Lajoie, ME Katt, EA Waters, BR Herrin, EV Shusta
Scientific Reports, 2022-04-11;12(1):6044.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Induction of broadly reactive influenza antibodies increases susceptibility to autoimmunity
Authors: JG Labombarde, MR Pillai, M Wehenkel, CY Lin, R Keating, SA Brown, JC Crawford, DC Brice, AH Castellaw, AH Mandarano, CS Guy, JR Mejia, CD Lewis, TC Chang, CM Oshansky, SS Wong, RJ Webby, M Yan, QZ Li, TN Marion, PG Thomas, MA McGargill
Cell Reports, 2022-03-08;38(10):110482.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot