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Recombinant Mouse IL-28A/IFN-lambda 2 Protein

Catalog #: 4635-ML
10 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
4635-ML-025/CF

With Carrier

Catalog # Availability Size / Price Qty
4635-ML-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (10)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL-28A/IFN-lambda 2 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The ED50 for this effect is 0.0800-0.800 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL-28A/IFN-lambda 2 protein
Asp20-Val193, with a C-terminal 6-His tag
Accession #
NP_001019844
N-terminal Sequence
Analysis
Asp20
Predicted Molecular Mass
20.5 kDa
SDS-PAGE
25 kDa and 30 kDa, reducing conditions

Product Datasheets

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4635-ML (with carrier)

Product Datasheet
COA
SDS

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4635-ML/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4635-ML

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

4635-ML/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-28A/IFN-lambda 2

IL-28A (also named interferon-lambda 2, IFN-lambda 2), IL-28B (IFN-lambda 3) and IL-29 (IFN-lambda 1) are type III interferons that are class II cytokine receptor ligands (1 - 4). They are distantly related to members of the IL-10 family and type I IFN family (1 - 4). Mouse IL-28A cDNA encodes a 193 amino acid (aa) protein with a 19 aa signal peptide and a 174 aa mature protein that lacks N-glycosylation sites. Mature mouse IL-28A shares 81% and 66% aa sequence identity with rat and human IL-28A, respectively, and functions across species (5). Mouse IL-28A and IL-28B share 97% aa identity; the mouse lacks a functional IL-29 gene (4). Type III interferons are widely expressed, but are mainly produced by antigen presenting cells in response to viruses and double-stranded RNA that interact with Toll-like receptors or RIG-1 family helicases (2 - 6). They signal through a widely expressed receptor that is a heterodimer of the IL-10 receptor  beta (IL-10 R beta ) and IL-28 receptor  alpha (IL-28 R alpha ; also called IFN-lambda  R1) (2, 3, 7, 9). Interaction of either type I or type III IFNs with their receptors activates similar pathways, including JAK tyrosine kinase activation, STAT phosphorylation and formation of the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex (1 - 3). Both type I and III IFNs induce antiviral activity and upregulate MHC class I antigen expression (2 - 6). Cell lines responsive to type III IFNs are also responsive to type I IFNs, but in general, higher concentrations of type III IFNs are needed for similar in vitro responses (8). In vivo, however, type III IFNs enhance levels of IFN-gamma in serum, suggesting that the robust antiviral activity of type III IFNs may stem in part from activation of the immune system (5, 7). Anti-proliferative and antitumor activity in vivo has also been shown for type III IFNs (9 - 11).

References
  1. Chen, Q. et al. (2006) Vitam. Horm. 74:207.
  2. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  3. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
  4. Bartlett, N.W. et al. (2005) J. Gen. Virol. 86:1589.
  5. Ank, N. et al. (2006) J. Virol. 80:4501.
  6. Onoguchi, K. et al. (2007) J. Biol. Chem. 282:7576.
  7. Siebler, J. et al. (2007) Gastroenterology 132:358.
  8. Meager, A. et al. (2005) Cytokine 31:109.
  9. Lasfar, A. et al. (2006) Cancer Res. 66:4468.
  10. Sato, A. et al. (2006) J. Immunol. 176:7686.
  11. Zitzmann, K. et al. (2006) Biochem. Biophys. Res. Commun. 344:1334.
Long Name
Interleukin 28A
Entrez Gene IDs
282616 (Human)
Alternate Names
interleukin-28A; IFN-lambda 2; IL28A; IL-28A; interferon, lambda 2

Citations for Recombinant Mouse IL-28A/IFN-lambda 2 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Synthetic mimetics assigned a major role to IFNAR2 in type I interferon signaling
    Authors: N Zoellner, N Coesfeld, FH De Vos, J Denter, HC Xu, E Zimmer, B Knebel, H Al-Hasani, S Mossner, PA Lang, DM Floss, J Scheller
    Frontiers in Microbiology, 2022-09-02;13(0):947169.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Inhaled delivery of recombinant interferon-lambda restores allergic inflammation after development of asthma by controlling Th2- and Th17-cell-mediated immune responses
    Authors: J Won, A Jo, CH Gil, S Kim, H Shin, H Jik Kim
    International immunopharmacology, 2022-08-27;112(0):109180.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Interferons limit autoantigen-specific CD8+ T-cell expansion in the non-obese diabetic mouse
    Authors: G Jhala, B Krishnamur, TC Brodnicki, T Ge, S Akazawa, C Selck, PM Trivedi, EG Pappas, L Mackin, N Principe, E Brémaud, DJ De George, L Boon, I Smyth, J Chee, TWH Kay, HE Thomas
    Cell Reports, 2022-04-26;39(4):110747.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Inhibiting Type I arginine methyltransferase activity promotes the T cell mediated antitumor immune response
    Authors: A Fedoriw, L Shi, S O'Brien, KN Smitheman, Y Wang, J Hou, C Sherk, S Rajapurkar, J Laraio, LJ Williams, C Xu, G Han, Q Feng, MT Bedford, L Wang, O Barbash, RG Kruger, P Hwu, HP Mohammad, W Peng
    Cancer Immunology Research, 2022-04-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Age-Dependent Effects of Type I and Type III IFNs in the Pathogenesis of Bordetella pertussis Infection and Disease
    Authors: J Ardanuy, K Scanlon, C Skerry, SY Fuchs, NH Carbonetti
    J. Immunol., 2020-03-09;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. Hypoxia and therapeutic treatment of EV-A71 with an immune modulator TLR7 agonist in a new immunocompetent mouse model
    Authors: AT Liou, CC Liao, SF Chou, YS Chang, CS Chang, C Shih
    J. Biomed. Sci., 2019-11-11;26(1):93.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. SIDT1 Localizes to Endolysosomes and Mediates Double-Stranded RNA Transport into the Cytoplasm
    Authors: TA Nguyen, BRC Smith, KD Elgass, SJ Creed, S Cheung, MD Tate, GT Belz, IP Wicks, SL Masters, KC Pang
    J. Immunol., 2019-05-06;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  8. STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection
    Authors: MT Stier, K Goleniewsk, JY Cephus, DC Newcomb, TP Sherrill, KL Boyd, MH Bloodworth, ML Moore, K Chen, JK Kolls, RS Peebles
    J. Immunol., 2017-06-02;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Bioassay
  9. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012-06-04;109(25):9971-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S
    J. Virol., 2010-09-29;84(24):12703-12.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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