Recombinant Mouse IL-24 (NS0-expressed) Protein Summary
Product Specifications
Gln66-Leu220
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7807-ML
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
7807-ML/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IL-24
Interleukin 24 (IL-24), also known as FISP (IL-4 induced secreted protein) in mouse, and mda-7 (melanoma differentiation associated gene‑7) in humans, is a member of the IL-10 family of helical cytokines (1-3). The mouse IL-24 gene encodes for a 181 amino acid (aa) precursor that includes a 26 aa signal sequence and an 18 kDa (predicted), 155 aa mature segment (4). Unlike mature human IL-24, which contains three potential N-linked glycosylation sites, mouse mature IL-24 has only one. In human, both glycosylation and the presence of an intrachain disulfide bond have been found to be necessary for full activity (5). This requirement for glycosylation is unique among IL-10 family members. Presumably, the same structural modifications apply to mouse IL-24. Mouse IL-24 is a 27 kDa protein when secreted, and is active on human receptors (1, 3). Mature mouse IL-24 shares 69%, 84%, 69%, 68% and 61% aa sequence identity with human, rat, equine, canine and bovine IL-24, respectively. A 119 aa isoform termed FISP-sp diverges at aa 77. FISP-sp has been found mainly in the endoplasmic reticulum of Th2 cells. It can dimerize with intracellular IL-24, and antagonize the pro-apoptotic effects of IL-24 (6). Under physiological conditions, cytokine-stimulated monocytes/macrophages and Th2 cells produce most secreted IL-24; other sources include B cells, keratinocytes, NK cells, and differentiating melanoma cells (1-3, 7-9). Secreted IL-24 binds and signals through complexes of IL‑20 R alpha :IL-20 R beta, or IL-22 R:IL-20 R beta (10). The IL-20 R alpha :IL-20 R beta complex is also a receptor for IL-19 and IL-20, while IL-22 R:IL-20 R beta binds IL‑20 (2, 10). Both receptors are widely expressed, but have not been found on hematopoietic cells (3). Even so, IL-24 induces type I proinflammatory cytokines in monocytes, and inhibits plasma cell differentiation in germinal center B cells (8, 11). The phenotype of mice transgenic for IL-24 is similar to that of IL-20 and IL-22 transgenic mice, indicating overlap in their activities, particularly in the skin (12). Secreted IL-24 is anti-angiogenic, binding receptors on endothelial cells and blocking their differentiation (13). Intratumoral injection of adenovirus coding for IL-24 causes tumor-specific apoptosis in humans, but it is not clear whether secreted IL-24 contributes to this effect (14).
- Schaefer, G. et al. (2001) J. Immunol. 166:5859.
- Trivella, D.B.B. et al. (2010) Cell. Mol. Life Sci. 67:2909.
- Wang, M. and P. Liang (2005) Immunology 114:166.
- Swissprot Accession # Q925J3.
- Fuson, K.L. et al. (2009) J. Biol. Chem. 284:30526.
- Sahoo, A. et al. (2008) J. Biol. Chem. 283:28860.
- Poindexter, N.J. et al. (2005) J. Leukoc. Biol. 78:745.
- Maarof, G. et al. (2010) Blood 115:1718.
- Poindexter, N.J. et al. (2010) Exp. Dermatol. 19:714.
- Wang, M. et al. (2002) J. Biol Chem. 227:7341.
- Caudell, E.G. et al. (2002) J. Immunol. 168:6041.
- He, M. and P. Liang (2010) J. Immunol. 184:1793.
- Ramesh, R. et al. (2003) Cancer Res. 63:5105.
- Emdad, L. et al. (2009) Cancer Biol. Ther. 8:391.
Citations for Recombinant Mouse IL-24 (NS0-expressed) Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Interleukin-24 regulates mucosal remodeling in inflammatory bowel diseases
Authors: A Ónody, A Veres-Szék, D Pap, R Rokonay, B Szebeni, E Sziksz, F Oswald, G Veres, Á Cseh, AJ Szabó, Á Vannay
Journal of Translational Medicine, 2021-06-02;19(1):237.
Species: Mouse
Sample Types:
Applications: In Vivo -
Murine astrocytes produce IL-24 and are susceptible to the immunosuppressive effects of this cytokine
Authors: AR Burmeister, MB Johnson, JJ Yaemmongko, I Marriott
J Neuroinflammation, 2019-03-02;16(1):55.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
IL-24 Promotes Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas
Authors: BX Ross, N Gao, X Cui, TJ Standiford, J Xu, FX Yu
J. Immunol, 2017-03-22;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
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