Recombinant Mouse IL-18 R beta/IL-1 R7 Fc Chimera, CF

Catalog #: 9459-AP Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9459-AP-100

Recombinant Mouse IL-18 R beta/IL-1 R7 Fc Chimera Bioactivity

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All IL-18 R beta/IL-1 R7 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL-18 R beta/IL-1 R7 Fc Chimera, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL‑18 R beta /IL‑1 R7 Fc Chimera is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL‑18/IL‑F4 (Catalog # 9139‑IL) that produces 50% optimal binding response is 1-6 μg/mL

Source

Mouse myeloma cell line, NS0-derived mouse IL-18 R beta/IL-1 R7 protein

Mouse IL-18 R beta /IL-1 R7 (Glu15 & Phe20-Asp357) Accession # XP_006495773	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_006495773

N-terminal Sequence
Analysis

Glu15, Phe20

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

66 kDa

SDS-PAGE

74-95 kDa, reducing conditions

Product Datasheets

9459-AP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9459-AP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Mouse IL-18 R beta /IL-1 R7 Fc Chimera (Catalog # 9459-AP) is coated at 2 µg/mL (100 µL /well), Recombinant Mouse IL-18/IL-1F4 (Catalog # 9139-IL) binds with an ED₅₀ of 1-6 µg/mL.

Reconstitution Calculator

Background: IL-18 R beta/IL-1 R7

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses (1). The functional IL-18 receptor complex consists of two components, the IL-18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness (1, 2). Mouse IL-18 R beta cDNA encodes a 614 amino acid (aa) residue precursor type I membrane protein with a 19 aa signal peptide, a 337 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 237 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon (1).

References

Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Wu, C., et al. (2003) J. Immunol. 273:5571.

Long Name

Interleukin 18 Receptor beta

Entrez Gene IDs

8807 (Human); 16174 (Mouse)

Alternate Names

Accessory protein-like; AcPL; CD218 antigen-like family member B; CD218b antigen; CD218b; CDw218b; IL-1 R7; IL-18 R beta; IL-18 receptor accessory protein; IL-18 receptor beta; IL18R beta; IL-18RAcP; IL18RAP; IL18RB; IL-18Rb; IL-18Rbeta; IL-18R-beta; IL-1R accessory protein-like; IL1R7; IL-1R7; IL-1R-7; IL-1RAcPL; interleukin 18 receptor accessory protein; Interleukin-1 receptor 7; interleukin-18 receptor accessory protein; Interleukin-18 receptor accessory protein-like; Interleukin-18 receptor beta; MGC120589; MGC120590