Recombinant Mouse IL-17RA/IL-17R Fc Chimera Protein, CF
Recombinant Mouse IL-17RA/IL-17R Fc Chimera Protein, CF Summary
Product Specifications
| Mouse IL-17 RA/IL-17 R (Ser32-Trp322) Accession # Q60943 |
IEGRMDP | Mouse IgG2A (Glu98-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4481-MR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: IL-17RA/IL-17R
IL-17 R, also known as IL-17 RA, is a 120 kDa type I transmembrane glycoprotein protein that plays a central role in inflammatory responses (1-3). Mature mouse IL‑17 R consists of a 291 amino acid (aa) extracellular domain, a 21 aa transmembrane segment, and a 521 aa cytoplasmic domain (4). The cytoplasmic domain contains a region homologous to the TIR domain of the TLR/IL-1 R family (5). Mouse IL-17 R shares 84% and 72% aa sequence identity with rat and human IL-17 R, respectively. Within the extracellular domain, it shares 18%-25% sequence identity with mouse IL-17 RB, C, D, and E. While the expression of IL-17 is restricted to activated T cells, IL-17 R exhibits a broad tissue distribution (4). Even in the absence of ligand, IL-17 R exists on the cell surface as a multimer (6). IL-17 R can bind IL-17 but must associate with IL-17 RC to transduce signals (7, 8). Interestingly, human IL-17 R does not appear to form productive complexes with mouse IL-17 RC (8). The IL-17 R can also signal in response to IL-17F (9). IL-17 R ligation promotes T cell activation and the production of IL-6, G-CSF, SCF, and multiple pro‑inflammatory chemokines (4, 7, 9, 10). IL-17A and IL-17F synergize with TNF-alpha in the induction of CXCL1, G-CSF, and IL-6 (9, 11). This effect requires the presence of both TNF RI and TNF RII (9). IL-17 interactions with IL-17 R also inhibit the TNF-alpha induced up-regulation of fibroblast CCL5 and VCAM-1 (11). CCL5 and VCAM-1 induced effects are differentially sensitive to blockade with IL-17 R specific antibodies, suggesting that IL-17 R triggers divergent intracellular signals (11). In vivo, IL‑17 R activity is important for increased generation of neutrophils and their recruitment to sites of inflammation (10, 12, 13). IL-17 R is required for host defense against microbial infection and for the progression of arthritis from inflammation to destructive joint erosion (10, 13).
- Iwakura, Y. and H. Ishigame (2006) J. Clin. Invest. 116:1218.
- Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
- Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
- Yao, Z. et al. (1995) Immunity 3:811.
- Novatchkova, M. et al. (2003) Trends Biochem. Sci. 28:226.
- Kramer, J.M. et al. (2006) J. Immunol. 176:711.
- Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
- Toy, D. et al. (2006) J. Immunol. 177:36.
- McAllister, F. et al. (2005) J. Immunol. 175:404.
- Ye, P. et al. (2001) J. Exp. Med. 194:519.
- Schnyder, B. et al. (2005) Cytokine 31:191.
- Tan, W. et al. (2006) J. Immunol. 176:6186.
- Lubberts, E. et al. (2005) J. Immunol. 175:3360.
Citation for Recombinant Mouse IL-17RA/IL-17R Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities.
Authors: Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL
J. Immunol., 2008-09-15;181(6):4299-310.
Species: Rat
Sample Types: In Vivo
Applications: Immunization
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