Home / IFN-kappa / Recombinant Mouse IFN-kappa Protein

Recombinant Mouse IFN-kappa Protein

Catalog #: 8437-MK
2 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
8437-MK-010/CF

With Carrier

Catalog # Availability Size / Price Qty
8437-MK-010
Recombinant Mouse IFN-kappa Protein Bioactivity
1 Image
Product Details
Citations (2)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IFN-kappa Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED50 for this effect is 0.2-1.2 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IFN-kappa protein
Leu22-Lys199
Accession #
Q7TSL0
N-terminal Sequence
Analysis
Leu22
Predicted Molecular Mass
21 kDa
SDS-PAGE
19-22 kDa, reducing conditions

Product Datasheets

You must select a language.

x

8437-MK (with carrier)

Product Datasheet
COA
SDS

You must select a language.

x

8437-MK/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8437-MK

Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Acetate, NaCl and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8437-MK/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Acetate, NaCl and EDTA.
Reconstitution Reconstitute at 200 μg/mL in sterile water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse IFN-kappa Protein Bioactivity View Larger

Recombinant Mouse IFN-kappa (Catalog # 8437-MK) exhibits anti-viral activity in L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.2-1.2 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IFN-kappa

Interferon (IFN)-kappa is a member of the type I IFN family, which also includes IFN-alpha, -beta, -epsilon, and -omega. Mouse IFN-kappa is expressed at low levels in peritoneal macrophages and its expression is up-regulated by double-stranded (ds) RNA and IFN-gamma (1). Mice over-expressing IFN-kappa in pancreatic beta  cells developed type I diabetes, similar to what has been reported for mice over-expressing IFN-alpha, -beta, and -gamma (1-4). Mouse IFN-kappa shares 68% and 30% amino acid sequence identity with rat and human IFN-kappa, respectively. Human IFN-kappa has been detected in keratinocytes, monocytes, and monocyte-derived dendritic cells and is reported to have contact-dependent antiviral activity (5-7). Human papillomavirus (HPV) 16 oncogene expression, which is necessary for the development of cervical cancer, has been shown to down-regulate human IFN-kappa expression (8-11).

References
  1. Vassileva, G. et al. (2003) J. Immunol. 170:5748.
  2. Stewart, T.A. et al. (1993) Science 260:1942.
  3. Sarvetnick, N. et al. (1988) Cell 52:773.
  4. Pelegrin, M. et al. (1998) J. Biol. Chem. 273:12332.
  5. LaFleur, D.W. et al. (2001) J. Biol. Chem. 276:39765.
  6. Nardelli, B. et al. (2002) J. Immunol. 169:4822.
  7. Buontempo, P.J. et al. (2006) J. Interferon Cytokine Res. 26:40.
  8. Rincon-Orozco, B. et al. (2009) Cancer Res. 69:8718.
  9. DeCarlo, C.A. et al. (2010) Lab. Invest. 90:1482.
  10. Reiser, J. et al. (2011) J. Virol. 85:11372.
  11. Sunthamala, N. et al. (2014) PLoS One 9:e91473.
Long Name
Interferon kappa
Entrez Gene IDs
56832 (Human); 387510 (Mouse)
Alternate Names
IFNK; IFNkappa; IFN-kappa

Citations for Recombinant Mouse IFN-kappa Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Trophoblastic signals facilitate endometrial interferon response and lipid metabolism, ensuring normal decidualization
    Authors: Yang, N;Sun, Y;Han, B;Deng, N;Li, G;Han, Q;Wang, Y;Cai, H;Liu, F;Cao, B;Deng, W;Bao, H;Kong, S;Lu, J;Wang, H;
    Cell reports
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo assay
  2. Interferon kappa (IFNk) is critical for normal wound repair and is decreased in diabetic wounds
    Authors: SJ Wolf, CO Audu, A Joshi, AD denDekker, WJ Melvin, FM Davis, X Xing, R Wasikowski, L Tsoi, S Kunkel, JE Gudjonsson, MX O'Riordan, JM Kahlenberg, KA Gallagher
    JCI Insight, 2022-05-09;0(0):.
    Species: Mouse
    Sample Types:
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Mouse IFN-kappa Protein

There are currently no reviews for this product. Be the first to review Recombinant Mouse IFN-kappa Protein and earn rewards!

Have you used Recombinant Mouse IFN-kappa Protein?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review