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Recombinant Mouse HGF Propeptide Protein, CF

Catalog #: 7058-HG
1 Citation Datasheet / COA / SDS
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7058-HG-010
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Recombinant Mouse HGF Propeptide Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
>40 ng of Recombinant Mouse HGF Propeptide is cleaved to release active HGF when treated for 4 hours at 37 °C with 10 ng of kallikrein.
Source
Spodoptera frugiperda, Sf 21 (stably transfected)-derived mouse HGF protein
Gln33-Leu728
Accession #
Q08048
N-terminal Sequence
Analysis
No results obtained: Gln33 predicted, sequencing might be blocked
Predicted Molecular Mass
79.3 kDa
SDS-PAGE
84 kDa, reducing conditions

Product Datasheets

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7058-HG

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7058-HG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HGF

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1-7). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive
disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta chain. Alternate splicing generates an additional HGF isoform with a 5 aa deletion in the first Kringle domain. Mouse Pro‑HGF shares 92%-93% aa sequence identity with bovine, canine, feline, and human Pro-HGF and 99% with rat Pro-HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (8, 9). HGF-dependent c-MET activation is implicated in the development of many human cancers (10). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (11, 12). HGF induces the up-regulation of Integrin  alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (13). This Integrin serves as a Collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (13, 14). HGF can also alter epithelium morphology by the induction of Nectin‑1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (15). In the thymus, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (16). HGF promotes the motility of cardiac stem cells in damaged myocardium (17).

References
  1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
  2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
  3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
  4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
  5. Nakamura, T. et al. (1989) Nature 342:440.
  6. Liu, Y. et al. (1993) Biochim. Biophys. Acta 1216:299.
  7. Lee, C.C. et al. (1993) Cell Adhes. Commun. 1:101.
  8. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
  9. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
  10. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  11. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
  12. Montesano, R. et al. (1991) Cell 67:901.
  13. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
  14. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
  15. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  16. Mineo, R. et al. (1994) Endocrinology 145:4355.
  17. Urbanek, K. et al. (2005) Circ. Res. 97:663.
Long Name
Hepatocyte Growth Factor
Entrez Gene IDs
3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 102133907 (Cynomolgus Monkey); 493705 (Feline)
Alternate Names
deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A

Citation for Recombinant Mouse HGF Propeptide Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Regulation of hepatocyte growth factor in mice with pneumonia by peptidases and trans-alveolar flux.
    Authors: Raymond W, Xu X, Nimishakavi S, Le C, McDonald D, Caughey G
    PLoS ONE, 2015-05-04;10(5):e0125797.
    Species: Mouse
    Sample Types: Protein
    Applications: Bioassay

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