Recombinant Mouse FGFR2 alpha (IIIb) Fc Chimera Protein, CF

Recombinant Mouse FGFR2 alpha (IIIb) Fc Chimera Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: FGFR2 alpha

Fibroblast growth factor receptor 2 alpha (FGF R2 alpha ) is a 140 kDa type I transmembrane receptor tyrosine kinase that mediates the heparan sulfate proteoglycan-dependent biological activity of several FGFs. It is a member of the FGF R family of proteins whose extracellular domains (ECD) contain three immunoglobulin (Ig)-like domains with a series of acidic residues between the first and second Ig-like domains (1, 2). Multiple forms of FGF R2 are generated by alternative splicing (1, 2). Alpha isoforms contain all three Ig-like domains, while beta forms lack the first Ig-like domain (3, 4). The C-terminal portion of the third Ig-like domain can be encoded by either of two mutually exclusive exons, giving rise to the IIIb/KGFR and IIIc/BEK isoforms with distinct ligand selectivity (3, 4). This recombinant protein corresponds to the ECD from Accession # ABL89196. It shares 94% amino acid sequence identity with the ECD of human FGF R2 alpha (IIIb). FGF R2 alpha (IIIb) signals in response to FGF-1, -3, -7, -10, and -22, leading to receptor dimerization and autophosphorylation of its tyrosine kinase domain (1, 2, 5, 6). In contrast, the IIIc isoform preferentially responds to FGF-1, -2, -4, -5, -6, -8, -9, -16, -17, -18, and -20 (5, 6). FGF R2 alpha (IIIb) is widely expressed on epithelial cells, while its ligands are typically expressed by neighboring mesenchymal cells (2, 7). Its involvement in epithelial-mesenchymal interactions plays an important role in the morphogenesis of many tissues. Knockout mice exhibit widespread developmental defects due to an unresponsiveness to normal mitogenic stimulation (8 - 11). Mutations in FGF R2 alpha (IIIb) as well as altered splicing patterns (to isoform IIIc) are associated with a variety of skin and skeletal disorders and the progression of many epithelial cancers (12 - 14).

Related Research Areas

• Cytokine and Growth Factor Receptors on VSMC
• FGF Family
• FGF Ligands
• FGF Regulators
• Glial Lineage Markers
• Receptor Tyrosine Kinases (RTKs)
• Receptor Tyrosine Kinases (RTKs) in the Akt Pathway
• Receptors in the Jak/STAT Pathway