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Recombinant Mouse FGF-23 Protein, CF

Catalog #: 2629-FG/CF
27 Citations Datasheet / COA / SDS
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2629-FG-025/CF
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Recombinant Mouse FGF-23 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human FGF RIIIc. The ED50 for this effect is 0.2‑1.2 µg/mL in the presence of Recombinant Mouse Klotho (Catalog # 1819-KL) and heparin.
Source
Mouse myeloma cell line, NS0-derived mouse FGF-23 protein
Tyr25-Val251 (Arg179Gln), with a C-terminal 6-His tag
Accession #
Q9EPC2
N-terminal Sequence
Analysis
Tyr25
Predicted Molecular Mass
26.1 kDa
SDS-PAGE
30-32 kDa, reducing conditions

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2629-FG/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2629-FG/CF

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: FGF-23

Fibroblast growth factor 23 (FGF-23) is a 30 - 32 kDa member of the FGF gene family. Based on its structure, it is further classified as an FGF19 subfamily member. This subfamily includes FGF-19, -21, and -23. Like all other FGF subfamilies, FGF-19 subfamily members contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Unlike other FGF subfamilies, FGF-19 subfamily members exist as highly diffusible molecules that is attributed to poor ECM/heparin sulfate binding (3, 4, 5, 6). The cDNA for mouse FGF-23 predicts a 251 aa polypeptide that contains a 24 aa signal sequence and a 227 aa mature region (7). Mature mouse FGF-23 shows 72% aa identity to human FGF-23 (8). The FGF-19 subfamily shares an unusual receptor configuration. The standard model for FGF signaling requires an FGF:FGFR:heparin sulfate complex. Given FGF-23’s minimal association with heparin, a substitute termed ( alpha -) Klotho has evolved that serves the same function. Although FGF-23 binds to the widely expressed “c” isoforms of FGFR1 and 3 plus FGFR4, Klotho has a restricted distribution that limits FGF-23 activity (10, 11, 12). It should be noted that heparin-dependency has been reported for FGF-19 signaling, and this observation may extend to FGF-23 (13). The FGF-19 subfamily is considered endocrine in nature. All three subfamily members impact some aspect of metabolism and all three are induced by a nuclear receptor heterodimer that includes the retinoid X receptor (14, 15, 16). FGF-23 is considered a phosphatonin; that is, a molecule that reduces circulating plasma phosphate. It is produced by osteocytes and osteoblasts in response to high circulating phosphate levels, elevated parathyroid hormone that induces hypercalcemia, and circulatory volume loading. Upon binding to FGF-23 receptors on renal proximal tubular epithelium, two basic changes are seen. First, the enzyme responsible for generating the active form of vitamin D is suppressed, resulting in decreased levels of bioactive vitamin D. Since vitamin D promotes intestinal phosphate absorption, plasma phosphate declines. Second, the transporters responsible for phosphate resorption on renal epithelium are down regulated, resulting in decreased uptake from urine and again a decline in blood phosphorus (17, 18).

References
  1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563. 
  2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  3. Fukumoto, S. (2007) Endocr. J. Sep 14; [Epub ahead of print].
  4. Huang, X. et al. (2006) Mol. Carcinog. 45:934. 
  5. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
  6. Harmer, N.J. et al. (2004) Biochemistry 43:629.
  7. Yamashita, T. et al. (2000) Biochem. Biophys. Res. Commun. 277:494.
  8. Shimada, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:6500.
  9. Kato, K. et al. (2006) J. Biol. Chem. 281:18370.
  10. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  11. Urakawa, I. et al. (2006) Nature 444:770.
  12. Hurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
  13. Wu, X. et al. (2007) J. Biol. Chem. 282:29069.
  14. Moore, D.D. (2007) Science 316:1436.
  15. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:7432.
  16. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
  17. Razzaque, M.S. and B. Lanske (2007) J. Endocrinol. 194:1.
  18. Liu, S. et al. (2007) Curr. Opin. Nephrol. Hypertens. 16:329.
Long Name
Fibroblast Growth Factor 23
Entrez Gene IDs
8074 (Human); 64654 (Mouse)
Alternate Names
ADHR; FGF23; FGF-23; fibroblast growth factor 23; HPDR2; HYPF; phosphatonin; PHPTC; tumor-derived hypophosphatemia inducing factor; Tumor-derived hypophosphatemia-inducing factor

Citations for Recombinant Mouse FGF-23 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Fibroblast Growth Factor (FGF) 23 and FGF Receptor 4 promote cardiac metabolic remodeling in chronic kidney disease
    Authors: Fuchs, MA;Burke, EJ;Latic, N;Murray, S;Li, H;Sparks, M;Abraham, D;Zhang, H;Rosenberg, P;Hänzelmann, S;Hausmann, F;Huber, T;Erben, R;Fisher-Wellman, K;Bursac, N;Wolf, M;Grabner, A;
    Research square
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  2. FGF23 directly inhibits osteoprogenitor differentiation in Dmp1 knockout mice
    Authors: Courbon, G;Kentrup, D;Thomas, JJ;Wang, X;Tsai, HH;Spindler, JJ;Von Drasek, J;Ndjonko, LM;Martinez-Calle, M;Lynch, S;Hivert, L;Wang, X;Chang, W;Feng, JQ;David, V;Martin, A;
    JCI insight
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Rapid genomic changes by mineralotropic hormones and kinase SIK inhibition drive coordinated renal Cyp27b1 and Cyp24a1 expression via CREB modules
    Authors: MB Meyer, NA Benkusky, SM Lee, SH Yoon, M Mannstadt, MN Wein, JW Pike
    The Journal of Biological Chemistry, 2022-09-30;0(0):102559.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Hyperphosphatemia increases inflammation to exacerbate anemia and skeletal muscle wasting independently of FGF23-FGFR4 signaling
    Authors: B Czaya, K Heitman, I Campos, C Yanucil, D Kentrup, D Westbrook, O Gutierrez, JL Babitt, G Jung, IB Salusky, M Hanudel, C Faul
    Elife, 2022-03-18;11(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  5. The anti-aging factor Klotho protects against acquired long QT syndrome induced by uremia and promoted by fibroblast growth factor 23
    Authors: JA Navarro-Ga, R Salguero-B, L González-L, L Martín-Nun, E Rodríguez-, T Bada-Bosch, E Hernández, E Mérida-Her, M Praga, J Solís, F Arribas, H Bueno, M Kuro-O, M Fernández-, LM Ruilope, C Delgado, G Ruiz-Hurta
    Bmc Medicine, 2022-01-19;20(1):14.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. Fibroblast Growth Factor 23 Stimulates Cardiac Fibroblast Activity through Phospholipase C-Mediated Calcium Signaling
    Authors: TW Lee, CC Chung, TI Lee, YK Lin, YH Kao, YJ Chen
    International Journal of Molecular Sciences, 2021-12-23;23(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Soluble Alpha-Klotho Alleviates Cardiac Fibrosis without Altering Cardiomyocytes Renewal
    Authors: WY Chen
    Int J Mol Sci, 2020-03-22;21(6):.
    Species: Rat
    Sample Types: cardiomyocyte
    Applications: Bioassay
  8. Fibroblast growth factor 8b induces uncoupling protein 1 expression in epididymal white preadipocytes
    Authors: S Westphal, T Gantert, C Kless, K Hüttinger, M Klingenspo, T Fromme
    Sci Rep, 2019-06-11;9(1):8470.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Targeted genomic deletions identify diverse enhancer functions and generate a kidney-specific, endocrine-deficient Cyp27b1 pseudo-null mouse
    Authors: MB Meyer, NA Benkusky, M Kaufmann, SM Lee, RR Redfield, G Jones, JW Pike
    J. Biol. Chem., 2019-05-03;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. Klotho controls the brain-immune system interface in the choroid plexus
    Authors: L Zhu, LR Stein, D Kim, K Ho, GQ Yu, L Zhan, TE Larsson, L Mucke
    Proc. Natl. Acad. Sci. U.S.A., 2018-11-09;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Twist1-Haploinsufficiency Selectively Enhances the Osteoskeletal Capacity of Mesoderm-Derived Parietal Bone Through Downregulation of Fgf23
    Authors: N Quarto, S Shailendra, NP Meyer, S Menon, A Renda, MT Longaker
    Front Physiol, 2018-10-15;9(0):1426.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  12. Regulation of vitamin D metabolizing enzymes in murine renal and extrarenal tissues by dietary phosphate, FGF23, and 1,25(OH)2D3
    Authors: L Kägi, C Bettoni, EM Pastor-Arr, U Schnitzbau, N Hernando, CA Wagner
    PLoS ONE, 2018-05-17;13(5):e0195427.
    Species: Mouse
    Sample Types:
    Applications: In Vivo
  13. FGF23 modulates the effects of erythropoietin on gene expression in renal epithelial cells
    Authors: M Yashiro, M Ohya, T Mima, Y Ueda, Y Nakashima, K Kawakami, Y Ishizawa, S Yamamoto, S Kobayashi, T Yano, Y Tanaka, K Okuda, T Sonou, T Shoshihara, Y Iwashita, Y Iwashita, K Tatsuta, R Matoba, S Negi, T Shigematsu
    Int J Nephrol Renovasc Dis, 2018-04-04;11(0):125-136.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Inhibition of fibroblast growth factor 23 (FGF23) signaling rescues renal anemia
    Authors: R Agoro, A Montagna, R Goetz, O Aligbe, G Singh, LM Coe, M Mohammadi, S Rivella, D Sitara
    FASEB J., 2018-02-26;0(0):fj201700667R.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  15. Fibroblast growth factor type 1 receptor stimulation of T-type Cachannels in sensory neurons requires the phosphatidylinositol 3-kinase and protein kinase A pathways, independently of Akt
    Authors: W Si, Y Zhang, K Chen, D Hu, Z Qian, S Gong, H Li, Y Hao, J Tao
    Cell. Signal., 2018-01-31;45(0):93-101.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Peripherally derived FGF21 promotes remyelination in the central nervous system
    Authors: M Kuroda, R Muramatsu, N Maedera, Y Koyama, M Hamaguchi, H Fujimura, M Yoshida, M Konishi, N Itoh, H Mochizuki, T Yamashita
    J. Clin. Invest., 2017-08-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. FGF23 is synthesised locally by renal tubules and activates injury-primed fibroblasts
    Authors: ER Smith, SJ Tan, SG Holt, TD Hewitson
    Sci Rep, 2017-06-13;7(1):3345.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Bioassay
  18. FGF23/FGFR4-mediated left ventricular hypertrophy is reversible
    Authors: A Grabner, K Schramm, N Silswal, M Hendrix, C Yanucil, B Czaya, S Singh, M Wolf, S Hermann, J Stypmann, GS Di Marco, M Brand, MJ Wacker, C Faul
    Sci Rep, 2017-05-16;7(1):1993.
    Species: Mouse, Rat
    Sample Types: Whole Cells, Whole Tissue
    Applications: Bioassay
  19. Fibroblast-growth factor 23 promotes terminal differentiation of ATDC5 cells
    Authors: M Guibert, A Gasser, H Kempf, A Bianchi
    PLoS ONE, 2017-04-13;12(4):e0174969.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Persistent fibroblast growth factor 23 signalling in the parathyroid glands for secondary hyperparathyroidism in mice with chronic kidney disease
    Authors: K Kawakami, A Takeshita, K Furushima, M Miyajima, I Hatamura, M Kuro-O, Y Furuta, K Sakaguchi
    Sci Rep, 2017-01-17;7(0):40534.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  21. FGF23 is endogenously phosphorylated in bone cells.
    Authors: Lindberg I, Pang H, Stains J, Clark D, Yang A, Bonewald L, Li K
    J Bone Miner Res, 2015-03-01;30(3):449-54.
    Applications: Bioassay
  22. Elevated fibroblast growth factor 23 exerts its effects on placenta and regulates vitamin D metabolism in pregnancy of Hyp mice.
    Authors: Ohata Y, Yamazaki M, Kawai M, Tsugawa N, Tachikawa K, Koinuma T, Miyagawa K, Kimoto A, Nakayama M, Namba N, Yamamoto H, Okano T, Ozono K, Michigami T
    J Bone Miner Res, 2014-07-01;29(7):1627-38.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  23. FGF23 promotes renal calcium reabsorption through the TRPV5 channel.
    Authors: Andrukhova O, Smorodchenko A, Egerbacher M, Streicher C, Zeitz U, Goetz R, Shalhoub V, Mohammadi M, Pohl E, Lanske B, Erben R
    EMBO J, 2014-01-16;33(3):229-46.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Bioassay
  24. FGF23 is a novel regulator of intracellular calcium and cardiac contractility in addition to cardiac hypertrophy.
    Authors: Touchberry C, Green T, Tchikrizov V, Mannix J, Mao T, Carney B, Girgis M, Vincent R, Wetmore L, Dawn B, Bonewald L, Stubbs J, Wacker M
    Am J Physiol Endocrinol Metab, 2013-02-26;304(8):E863-73.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  25. Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet.
    Authors: Lau, Wei Ling, Leaf, Elizabet, Hu, Ming Cha, Takeno, Marc M, Kuro-o, Makoto, Moe, Orson W, Giachelli, Cecilia
    Kidney Int, 2012-08-29;82(12):1261-70.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  26. FGF23 induces left ventricular hypertrophy.
    Authors: Faul C, Amaral AP, Oskouei B, Hu MC, Sloan A, Isakova T, Gutierrez OM, Aguillon-Prada R, Lincoln J, Hare JM, Mundel P, Morales A, Scialla J, Fischer M, Soliman EZ, Chen J, Go AS, Rosas SE, Nessel L, Townsend RR, Feldman HI, St John Sutton M, Ojo A, Gadegbeku C, Di Marco GS, Reuter S, Kentrup D, Tiemann K, Brand M, Hill JA, Moe OW, Kuro-o M, Kusek JW, Keane MG, Wolf M
    J. Clin. Invest., 2011-10-10;121(11):4393-408.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  27. Parathyroid cell resistance to fibroblast growth factor 23 in secondary hyperparathyroidism of chronic kidney disease.
    Authors: Galitzer H, Ben-Dov IZ, Silver J, Naveh-Many T
    Kidney Int., 2009-12-16;77(3):211-8.
    Species: Rat
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo

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