Recombinant Mouse Fas/TNFRSF6/CD95 Fc Chimera Protein
Recombinant Mouse Fas/TNFRSF6/CD95 Fc Chimera Protein Summary
Product Specifications
| Mouse FAS (Gly14 - Arg169) Accession # P25446 |
DIEGRMD | Human IgG1 (Pro100 - Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
435-FA
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
435-FA/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Fas/TNFRSF6/CD95
Fas, also known as APO‑1 or CD95, belongs to the death receptor subfamily of the TNF receptor superfamily and is designated TNFRSF6 (1 ‑ 3). Mouse Fas cDNA encodes 327 amino acids (aa) that include a 21 aa signal peptide, a 148 aa extracellular domain (ECD) that contains three cysteine‑ich TNFR repeats, a 17 aa transmembrane sequence, and a 141 aa cytoplasmic domain containing a death domain (DD), which is required for transducing apoptotic signals (4). Mature mouse Fas ECD shares 68% aa sequence identity with rat Fas, and 53 ‑ 55% with human, feline, bovine and porcine Fas. One potentially secreted 63 aa isoform diverges at aa 61 (5). The Fas ligand (FasL, TNFSF6) is a type II transmembrane protein of the TNF family that can be expressed on activated T‑lymphocytes, NK cells and cells in immune privileged sites, or shed in soluble form (2). Polymorphisms of FasL impart differing activity of Fas depending on mouse strain (6). Engagement of FAS induces oligomerization of preformed Fas trimers (1, 2). The activated receptor recruits the adaptor molecule FADD to form the Death‑Inducing Signaling Complex (DISC). Upon activation, caspases in the DISC initiate the apoptotic signaling cascade (7). Fas is prominent in epithelial cells, hepatocytes, activated mature lymphocytes, virus‑transformed lymphocytes and tumor cells. It is an essential mediator in the activation‑induced death of T lymphocytes that terminates the immune reaction (1, 2, 8). In immune‑privileged tissues, infiltrating Fas‑bearing lymphocytes and inflammatory cells are killed by FasL engagement (9). Both humans and mice with genetic defects in Fas accumulate abnormal lymphocytes and develop systemic autoimmunity (1 ‑ 3). The Fas pathway also appears to cross‑communicate with the BIM (mitochondrial/intrinsic) apoptosis pathway (1).
- Bouillet, P. and L.A. O’Reilly (2009) Nat. Rev. Immunol. 9:514.
- Strasser, A. et al. (2009) Immunity 30:180.
- Ashkenazi, A. and V. Dixit (1999) Curr. Opin. Cell Biol. 11:255.
- SwissProt accession P25446.
- NCBI accession NP_001140180.
- Kayagaki, N. et al. (1997) Proc. Natl. Acad. Sci. USA 94:3914.
- Thorburn, A. (2003) Cellular Signaling 16:139.
- Barreiro, R. et al. (2004) J. Immunol. 173:1519.
- Ferguson, T.A. and T.S Griffith (2006) Immunol. Rev. 213:228.
Citations for Recombinant Mouse Fas/TNFRSF6/CD95 Fc Chimera Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Targeting necroptosis in muscle fibers ameliorates inflammatory myopathies
Authors: M Kamiya, F Mizoguchi, K Kawahata, D Wang, M Nishibori, J Day, C Louis, IP Wicks, H Kohsaka, S Yasuda
Nature Communications, 2022-01-10;13(1):166.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Signaling by the Epstein-Barr virus LMP1 protein induces potent cytotoxic CD4+ and CD8+ T cell responses
Authors: IK Choi, Z Wang, Q Ke, M Hong, Y Qian, X Zhao, Y Liu, HJ Kim, J Ritz, H Cantor, K Rajewsky, KW Wucherpfen, B Zhang
Proc. Natl. Acad. Sci. U.S.A., 2018-01-08;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Deficiency in the mitochondrial apoptotic pathway reveals the toxic potential of autophagy under ER stress conditions.
Authors: Deegan S, Saveljeva S, Logue S, Pakos-Zebrucka K, Gupta S, Vandenabeele P, Bertrand M, Samali A
Autophagy, 2014-01-01;10(11):1921-36.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Tissue inhibitor of metalloproteinases-3 facilitates Fas-mediated neuronal cell death following mild ischemia.
Authors: Wetzel M, Li L, Harms KM, Roitbak T, Ventura PB, Rosenberg GA, Khokha R, Cunningham LA
Cell Death Differ., 2007-10-26;15(1):143-51.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Evidence for a role for notch signaling in the cytokine-dependent survival of activated T cells.
Authors: Bheeshmachar G, Purushotaman D, Sade H, Gunasekharan V, Rangarajan A, Sarin A
J. Immunol., 2006-10-15;177(8):5041-50.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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