Recombinant Mouse EphA2 Fc Chimera Protein, CF Summary
Product Specifications
Immobilized Recombinant Mouse EphA2 Fc Chimera at 2 µg/mL (100 µL/well) can bind Recombinant Mouse Ephrin-A1 Fc Chimera with a linear range of 0.078-5 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
| Mouse EphA2 (Ala22-Ala535) Accession # AAA82113 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
639-A2
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-HCl and EDTA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: EphA2
EphA2, also known as Eck, Myk2, and Sek2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA2 has been shown to bind ephrin-A3, ephrin-A1, ephrin-A5, ephrin-A4, and ephrin-A2 (2, 3). The extracellular domains of mouse and human EphA2 share greater than 92% amino acid identity. Only membrane-bound or Fc‑clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).
- Eph Nomenclature Committee [letter] (1997) Cell 90:403.
- Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
- Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.
Citations for Recombinant Mouse EphA2 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells
Authors: S Kohara, K Ogawa
Biomedicines, 2022-12-12;10(12):.
Species: Mouse
Sample Types: Tissue Lysate
Applications: Bioassay -
EphA2 contributes to disruption of the blood-brain barrier in cerebral malaria
Authors: TK Darling, PN Mimche, C Bray, B Umaru, LM Brady, C Stone, CE Eboumbou M, TE Lane, LS Ayong, TJ Lamb
PLoS Pathog., 2020-01-30;16(1):e1008261.
Species: Mouse
Sample Types: In Vivo, Whole Cell
Applications: Bioassay, In Vivo -
Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2
Authors: M Takasugi, R Okada, A Takahashi, D Virya Chen, S Watanabe, E Hara
Nat Commun, 2017-06-06;8(0):15729.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Bioassay -
High Affinity Binders to EphA2 Isolated from Abdurin Scaffold Libraries; Characterization, Binding and Tumor Targeting.
Authors: Ullman C, Mathonet P, Oleksy A, Diamandakis A, Tomei L, Demartis A, Nardi C, Sambucini S, Missineo A, Alt K, Hagemeyer C, Harris M, Hedt A, Weis R, Gehlsen K
PLoS ONE, 2015-08-27;10(8):e0135278.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
ADAM12-cleaved ephrin-A1 contributes to lung metastasis.
Authors: Ieguchi K, Tomita T, Omori T, Komatsu A, Deguchi A, Masuda J, Duffy S, Coulthard M, Boyd A, Maru Y
Oncogene, 2013-05-20;33(17):2179-90.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Lithocholic acid is an Eph-ephrin ligand interfering with Eph-kinase activation.
Authors: Giorgio C, Hassan Mohamed I, Flammini L, Barocelli E, Incerti M, Lodola A, Tognolini M
PLoS ONE, 2011-03-30;6(3):e18128.
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay -
Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling.
Authors: Afshari FT, Kwok JC, Fawcett JW
J. Neurosci., 2010-03-24;30(12):4246-55.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
EphA2 receptor mediates increased vascular permeability in lung injury due to viral infection and hypoxia.
Authors: Cercone MA, Schroeder W, Schomberg S, Carpenter TC
Am. J. Physiol. Lung Cell Mol. Physiol., 2009-08-14;297(5):L856-63.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
A human antibody-drug conjugate targeting EphA2 inhibits tumor growth in vivo.
Authors: Jackson D, Gooya J, Mao S, Kinneer K, Xu L, Camara M, Fazenbaker C, Fleming R, Swamynathan S, Meyer D, Senter PD, Gao C, Wu H, Kinch M, Coats S, Kiener PA, Tice DA
Cancer Res., 2008-11-15;68(22):9367-74.
Applications: Binding Assay -
Antiangiogenic and antitumor efficacy of EphA2 receptor antagonist.
Authors: Dobrzanski P, Hunter K, Jones-Bolin S, Chang H, Robinson C, Pritchard S, Zhao H, Ruggeri B
Cancer Res., 2004-02-01;64(3):910-9.
Species: Mouse, Rat
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo
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