Recombinant Mouse EPCR Protein, CF Summary
Product Specifications
Leu18-Ser214, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9068-ER
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: EPCR
The endothelial protein C receptor (EPCR), also known as CD201, is an approximately 50 kDa transmembrane glycoprotein expressed on vascular endothelial cells and functions as a negative regulator of thrombosis (1). Mature mouse EPCR consists of a 197 amino acid extracellular domain (ECD), a 21 aa transmembrane segment, and a 7 aa cytoplasmic tail (2). Within the ECD, mouse EPCR shares 65% and 85% aa sequence identity with human and rat EPCR, respectively. EPCR inhibits thrombosis through its interactions with Protein C, activated Protein C (APC), and Coagulation Factors VII, and VIIa (3, 4). It enhances the activation of Protein C in response to complexes of Thrombin-Thrombomodulin (5). In humans, a soluble form of EPCR can be produced by alternative splicing or ADAM17/TACE mediated shedding (6-9), and this protein inhibits the anti-coagulant activity of APC (10, 11). EPCR can be degraded on the surface of endothelial cells by Neutrophil Elastase (12). Activation of EPCR also protects vascular endothelial cells from Thrombin-induced apoptosis (13). EPCR binds to CD11b/CD18 (Mac-1) on monocytes and mediates monocyte adhesion to the vascular endothelium (14). In addition, EPCR binds to the antigen receptor on gamma δ T cells (15), promotes hematopoietic stem cell retention in the bone marrow (9), and binds to surface proteins of some species of Plasmodium, contributing to pathogenicity in severe malaria (16).
- Montes, R. et al. (2012) Thromb. Haemost. 107:815.
- Fukudome, K. and C.T. Esmon (1995) J. Biol. Chem. 270:5571.
- Fukudome, K. and C.T. Esmon (1994) J. Biol. Chem. 269:26486.
- Ghosh, S. et al. (2007) J. Biol. Chem. 282:11849.
- Stearns-Kurosawa, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:10212.
- Saposnik, B. et al. (2008) Blood 111:3442.
- Qu, D. et al. (2007) J. Thromb. Haemost. 5:395.
- Xu, J. et al. (2000) J. Biol. Chem. 275:6038.
- Gur-Cohen, S. et al. (2015) Nat. Med. 21:1307.
- Kurosawa, S. et al. (1997) J. Clin. Invest. 100:411.
- Liaw, P.C. et al. (2000) J. Biol. Chem. 275:5447.
- Villegas-Mendez, A. et al. (2007) J. Thromb. Haemost. 5:980.
- Bae, J.-S. et al. (2007) Blood 110:3909.
- Fink, K. et al. (2013) PLoS One 8:e53103.
- Willcox, C.R. et al. (2012) Nat. Immunol. 13:872.
- Turner, L. et al. (2013) Nature 498:502.
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