Recombinant Mouse ELFN1 His-tag Protein, CF Summary
Product Specifications
Gln26-Tyr418, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10644-EF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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2 μg/lane of Recombinant Mouse ELFN1 His-tag Protein (Catalog # 10644-EF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 73-84 kDa.
Reconstitution Calculator
Background: ELFN1
Extracellular leucine-rich repeat and fibronectin type-III domain-containing protein 1 (ELFN1), also known as protein phosphatase 1 regulatory subunit 28 (PPP1R28), is a member of the large family of leucin-rich repeat (LRR) neuronal adhesion protein (1,2). Mouse ELFN1 consists of an extracellular domain (ECD) containing five LRR domains, one LRR C-terminal (LRRCT) domain and an FN3 domain, a type I transmembrane domain, and a cytoplasmic tail (2). Within the mature ECD, mouse ELFN1 shares 89% and 100% amino acid sequence identity with human and rat ELFN1, respectively. ELFN1 is strongly expressed in globus pallidus and interneurons in cortex and hippocampus in both developing and adult brains and is a selective binding partner of group III glutamate receptors (mGluR) (2, 3). Recent studies showed that ELFN1 physically anchor metabotropic glutamate receptor 6 (mGluR6) and mGluR7 across retinal and hippocampal synapses (3, 4), and can be recruited selectively to all group III mGluRs (mGluR4, mGluR6, mGluR7, and mGluR8) to allosterically modulate these receptors (2). ELFN1 mutant mice display seizures, subtle motor abnormalities, hyperactivity and altered thigmotaxis behavior (4). Given the functions and discrete patterns of many known LRR family proteins it has been proposed that ELFN1 could serve as a neuronal adhesion molecule and play an integral role in synapse formation and differentiation via the coordination of both pre- and postsynaptic machineries, thereby involved in neurite outgrowth, axon guidance, fasciculation, and synapse formation (5).
- Stachniak, T. et al. (2019) J. Neurosci. 39:4461.
- Dunn, H.A. et al. (2018) Proc. Natl. Acad. Sci. USA 115:5022.
- Dolan, J. et al. (2007) BMC Genomics 8:320.
- Dolan, J. and Mitchel, K.J. (2013) PLoS One 8:e8049.
- Williams, M.E. et al. (2010) Neuron 68:9.
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