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Recombinant Mouse E-Selectin/CD62E Fc Chimera Protein, CF

Catalog #: 575-ES
43 Citations 2 Reviews Datasheet / COA / SDS
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575-ES-100
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Recombinant Mouse E-Selectin/CD62E Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to mouse E-Selectin/Fc Chimera coated plates (2 µg/mL with 100 µL/well), approximately 85-100% will adhere for 1 hour incubation at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse E-Selectin/CD62E protein
Mouse E-Selectin
(Trp22-Pro557)
Accession # Q00690		 IEGRMD Human IgG1
(Pro100-Lys330) 		6-His tag
N-terminus C-terminus
Accession #
Q00690
N-terminal Sequence
Analysis
Trp22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
85.8 kDa (monomer)
SDS-PAGE
110 - 130 kDa, reducing conditions

Product Datasheets

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575-ES

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

575-ES

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: E-Selectin/CD62E

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouse E‑Selectin proteins share 81% amino acid similarity.

E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

Entrez Gene IDs
6401 (Human); 20339 (Mouse); 25544 (Rat); 102143564 (Cynomolgus Monkey)
Alternate Names
CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E

Citations for Recombinant Mouse E-Selectin/CD62E Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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  1. E-selectin-mediated rapid NLRP3 inflammasome activation regulates S100A8/S100A9 release from neutrophils via transient gasdermin D pore formation
    Authors: Pruenster, M;Immler, R;Roth, J;Kuchler, T;Bromberger, T;Napoli, M;Nussbaumer, K;Rohwedder, I;Wackerbarth, LM;Piantoni, C;Hennis, K;Fink, D;Kallabis, S;Schroll, T;Masgrau-Alsina, S;Budke, A;Liu, W;Vestweber, D;Wahl-Schott, C;Roth, J;Meissner, F;Moser, M;Vogl, T;Hornung, V;Broz, P;Sperandio, M;
    Nature immunology
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Synergistic effect of tumor chemo-immunotherapy induced by leukocyte-hitchhiking thermal-sensitive micelles
    Authors: J Qi, F Jin, Y You, Y Du, D Liu, X Xu, J Wang, L Zhu, M Chen, G Shu, L Wu, J Ji, Y Du
    Nature Communications, 2021-08-06;12(1):4755.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Interferon Gamma Mediates Hematopoietic Stem Cell Activation and Niche Relocalization through BST2
    Authors: MA Florez, KA Matatall, Y Jeong, L Ortinau, PW Shafer, AM Lynch, R Jaksik, M Kimmel, D Park, KY King
    Cell Reports, 2020-12-22;33(12):108530.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Neutrophil expressed CD47 regulates CD11b/CD18-dependent neutrophil transepithelial migration in the intestine in vivo
    Authors: V Azcutia, M Kelm, AC Luissint, K Boerner, S Flemming, M Quiros, G Newton, A Nusrat, FW Luscinskas, CA Parkos
    Mucosal Immunol, 2020-06-19;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Endothelial E-selectin inhibition improves acute myeloid leukaemia therapy by disrupting vascular niche-mediated chemoresistance
    Authors: V Barbier, J Erbani, C Fiveash, JM Davies, J Tay, MR Tallack, J Lowe, JL Magnani, DR Pattabiram, AC Perkins, J Lisle, JEJ Rasko, JP Levesque, IG Winkler
    Nat Commun, 2020-04-27;11(1):2042.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Efficient homing of T cells via afferent lymphatics requires mechanical arrest and integrin-supported chemokine guidance
    Authors: R Martens, M Permanyer, K Werth, K Yu, A Braun, O Halle, S Halle, GE Patzer, B Bošnjak, F Kiefer, A Janssen, M Friedrichs, J Poetzsch, K Kohli, Y Lueder, R Gutierrez, N Eckert, T Worbs, M Galla, R Förster
    Nat Commun, 2020-02-28;11(1):1114.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  7. Functional Binding of E-selectin to its Ligands is Enhanced by Structural Features Beyond its Lectin Domain
    Authors: FA Aleisa, K Sakashita, JM Lee, DB AbuSamra, B AlAlwan, S Nozue, M Tehseen, SM Hamdan, S Habuchi, T Kusakabe, JS Merzaban
    J. Biol. Chem., 2020-01-16;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Targeted Expansion of Tissue-Resident CD8+ T Cells to Boost Cellular Immunity in the Skin
    Authors: SJ Hobbs, JC Nolz
    Cell Rep, 2019-12-03;29(10):2990-2997.e2.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Glycoengineering of Chimeric Antigen Receptor (CAR) T-cells to Enforce E-selectin Binding
    Authors: N Mondal, M Silva, AP Castano, MV Maus, R Sackstein
    J. Biol. Chem., 2019-10-18;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  10. Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues
    Authors: EW Martin, R Malgor, VA Resto, DJ Goetz, MM Burdick
    Sci Rep, 2019-06-11;9(1):8511.
    Species: Mouse
    Sample Types: Protein
    Applications: Bioassay
  11. The vascular bone marrow niche influences outcome in chronic myeloid leukemia via the E-selectin - SCL/TAL1 - CD44 axis
    Authors: PS Godavarthy, R Kumar, SC Herkt, RS Pereira, N Hayduk, ES Weissenber, D Aggoune, Y Manavski, T Lucas, KT Pan, JM Voutsinas, Q Wu, MC Müller, S Saussele, T Oellerich, VG Oehler, J Lausen, DS Krause
    Haematologica, 2019-04-24;0(0):.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. CD4 T cell epitope specificity and cytokine potential are preserved as cells transition from the lung vasculature to lung tissue following influenza infection
    Authors: A DiPiazza, N Laniewski, A Rattan, DJ Topham, J Miller, AJ Sant
    J. Virol., 2018-06-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Staining of E-selectin ligands on paraffin-embedded sections of tumor tissue
    Authors: MA Carrascal, C Talina, P Borralho, A Gonçalo Mi, AR Henriques, C Pen, M Martins, S Braga, R Sackstein, PA Videira
    BMC Cancer, 2018-05-02;18(1):495.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice
    Authors: K Morita, C Tokushige, S Maeda, H Kiyose, M Noura, A Iwai, M Yamada, G Kashiwazak, J Taniguchi, T Bando, M Hirata, TR Kataoka, T Nakahata, S Adachi, H Sugiyama, Y Kamikubo
    Blood Adv, 2018-03-13;2(5):509-515.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression
    Authors: C Donnelly, B Dykstra, N Mondal, J Huang, BJ Kaskow, R Griffin, R Sackstein, C Baecher-Al
    Sci Rep, 2018-01-11;8(1):420.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Galectin-3: A Positive Regulator of Leukocyte Recruitment in the Inflamed Microcirculation
    Authors: BR Gittens, JV Bodkin, S Nourshargh, M Perretti, D Cooper
    J. Immunol., 2017-04-24;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Survival of tissue-resident memory T cells requires exogenous lipid uptake and metabolism
    Authors: Y Pan, T Tian, CO Park, SY Lofftus, S Mei, X Liu, C Luo, JT O'Malley, A Gehad, JE Teague, SJ Divito, R Fuhlbrigge, P Puigserver, JG Krueger, GS Hotamislig, RA Clark, TS Kupper
    Nature, 2017-02-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Dermal ?� T Cells Do Not Freely Re-Circulate Out of Skin and Produce IL-17 to Promote Neutrophil Infiltration during Primary Contact Hypersensitivity
    Authors: X Jiang, CO Park, J Geddes Swe, MJ Yoo, O Gaide, TS Kupper
    PLoS ONE, 2017-01-12;12(1):e0169397.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Rac GTPase Activating Protein ARHGAP25 Regulates Leukocyte Transendothelial Migration in Mice
    J Immunol, 2016-08-26;0(0):.
    Species: Mouse
    Sample Types: Whole Blood
    Applications: Bioassay
  20. Leukocyte-borne alpha(1,3)-fucose is a negative regulator of beta2-integrin-dependent recruitment in lung inflammation.
    Authors: Buffone A, Nasirikenari M, Manhardt C, Lugade A, Bogner P, Thanavala Y
    J Leukoc Biol, 2016-08-26;101(2):459-470.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. CD43 Functions as an E-Selectin Ligand for Th17 Cells In Vitro and Is Required for Rolling on the Vascular Endothelium and Th17 Cell Recruitment during Inflammation In Vivo.
    Authors: Velazquez F, Grodecki-Pena A, Knapp A, Salvador A, Nevers T, Croce K, Alcaide P
    J Immunol, 2015-12-23;196(3):1305-16.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. HCELL Expression on Murine MSC Licenses Pancreatotropism and Confers Durable Reversal of Autoimmune Diabetes in NOD Mice.
    Authors: Abdi R, Moore R, Sakai S, Donnelly C, Mounayar M, Sackstein R
    Stem Cells, 2015-05-01;33(5):1523-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Glycopeptide analogues of PSGL-1 inhibit P-selectin in vitro and in vivo.
    Authors: Krishnamurthy V, Sardar M, Ying Y, Song X, Haller C, Dai E, Wang X, Hanjaya-Putra D, Sun L, Morikis V, Simon S, Woods R, Cummings R, Chaikof E
    Nat Commun, 2015-03-31;6(0):6387.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Mutation in the CD45 inhibitory wedge modulates integrin activation and leukocyte recruitment during inflammation.
    Authors: Germena G, Volmering S, Sohlbach C, Zarbock A
    J Immunol, 2014-12-10;194(2):728-38.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Raised soluble P-selectin moderately accelerates atherosclerotic plaque progression.
    Authors: Woollard K, Lumsden N, Andrews K, Aprico A, Harris E, Irvine J, Jefferis A, Fang L, Kanellakis P, Bobik A, Chin-Dusting J
    PLoS ONE, 2014-05-20;9(5):e97422.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  26. A circadian clock gene, Rev-erbalpha, modulates the inflammatory function of macrophages through the negative regulation of Ccl2 expression.
    Authors: Sato S, Sakurai T, Ogasawara J, Takahashi M, Izawa T, Imaizumi K, Taniguchi N, Ohno H, Kizaki T
    J Immunol, 2013-12-04;192(1):407-17.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Human fucosyltransferase 6 enables prostate cancer metastasis to bone.
    Authors: Li J, Guillebon A, Hsu J, Barthel S, Dimitroff C, Lee Y, King M
    Br J Cancer, 2013-10-31;109(12):3014-22.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Bioassay
  28. CXCL9 causes heterologous desensitization of CXCL12-mediated memory T lymphocyte activation.
    Authors: Giegold O, Ogrissek N, Richter C, Schroder M, Herrero San Juan M, Pfeilschifter J, Radeke H
    J Immunol, 2013-02-27;190(7):3696-705.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Difference in Th1 and Th17 lymphocyte adhesion to endothelium.
    Authors: Alcaide P, Maganto-Garcia E, Newton G, Travers R, Croce KJ, Bu DX, Luscinskas FW, Lichtman AH
    J. Immunol., 2012-01-04;188(3):1421-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Efficient induction of CCR9 on T cells requires coactivation of retinoic acid receptors and retinoid X receptors (RXRs): exaggerated T Cell homing to the intestine by RXR activation with organotins.
    Authors: Takeuchi H, Yokota A, Ohoka Y, Kagechika H, Kato C, Song SY, Iwata M
    J. Immunol., 2010-09-29;185(9):5289-99.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
    Authors: Kato N, Yuzawa Y, Kosugi T
    J. Am. Soc. Nephrol., 2009-05-14;20(7):1565-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Binding Assay
  32. Phosphatidylinositol-3-OH kinase and nutrient-sensing mTOR pathways control T lymphocyte trafficking.
    Authors: Sinclair LV, Finlay D, Feijoo C, Cornish GH, Gray A, Ager A, Okkenhaug K, Hagenbeek TJ, Spits H, Cantrell DA
    Nat. Immunol., 2008-04-06;9(5):513-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. The metastatic T-cell hybridoma antigen/P-selectin glycoprotein ligand 1 is required for hematogenous metastasis of lymphomas.
    Authors: Raes G, Ghassabeh GH, Brys L, Mpofu N, Verschueren H, Vanhecke D, De Baetselier P
    Int. J. Cancer, 2007-12-15;121(12):2646-52.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. Vitamins A and D are potent inhibitors of cutaneous lymphocyte-associated antigen expression.
    Authors: Yamanaka K, Dimitroff CJ, Fuhlbrigge RC, Kakeda M, Kurokawa I, Mizutani H, Kupper TS
    J. Allergy Clin. Immunol., 2007-10-01;121(1):148-157.e3.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot (Detection)
  35. The 130-kDa glycoform of CD43 functions as an E-selectin ligand for activated Th1 cells in vitro and in delayed-type hypersensitivity reactions in vivo.
    Authors: Alcaide P, King SL, Dimitroff CJ, Lim YC, Fuhlbrigge RC, Luscinskas FW
    J. Invest. Dermatol., 2007-03-29;127(8):1964-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Chondroitin sulfate glycosaminoglycans as major P-selectin ligands on metastatic breast cancer cell lines.
    Authors: Monzavi-Karbassi B, Stanley JS, Hennings L, Jousheghany F, Artaud C, Shaaf S, Kieber-Emmons T
    Int. J. Cancer, 2007-03-15;120(6):1179-91.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Varied levels of reactivity by different E-selectin/Fc constructs with cutaneous lymphocyte-associated antigen (CLA)(+) CD4(+) T cells.
    Authors: Ni</LastName><ForeName>Zh Z</Initial, Ni Z, Walcheck B
    Immunol. Lett., 2007-01-03;108(2):179-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. The monoclonal antibody CHO-131 identifies a subset of cutaneous lymphocyte-associated antigen T cells enriched in P-selectin-binding cells.
    Authors: Ni Z, Campbell JJ, Niehans G, Walcheck B
    J. Immunol., 2006-10-01;177(7):4742-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  39. Requirement for CD44 in homing and engraftment of BCR-ABL-expressing leukemic stem cells.
    Authors: Krause DS, Lazarides K, von Andrian UH, Van Etten RA
    Nat. Med., 2006-09-24;12(10):1175-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  40. Dynamic programming of CD8+ T cell trafficking after live viral immunization.
    Authors: Liu L, Fuhlbrigge RC, Karibian K, Tian T, Kupper TS
    Immunity, 2006-09-01;25(3):511-20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  41. A down-regulatable E-selectin ligand is functionally important for PSGL-1-independent leukocyte-endothelial cell interactions.
    Authors: Zanardo RC, Bonder CS, Hwang JM, Andonegui G, Liu L, Vestweber D, Zbytnuik L, Kubes P
    Blood, 2004-08-10;104(12):3766-73.
    Species: Mouse
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  42. Dendritic cell immunization route determines CD8+ T cell trafficking to inflamed skin: role for tissue microenvironment and dendritic cells in establishment of T cell-homing subsets.
    Authors: Dudda JC, Simon JC, Martin S
    J. Immunol., 2004-01-15;172(2):857-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  43. CXCR4 enhances adhesion of B16 tumor cells to endothelial cells in vitro and in vivo via beta(1) integrin.
    Authors: Cardones AR, Murakami T, Hwang ST
    Cancer Res., 2003-10-15;63(20):6751-7.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Recombinant Mouse E-Selectin/CD62E Fc Chimera Protein, CF

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Recombinant Mouse E-Selectin/CD62E Fc Chimera Protein, CF
By Mingxia Zhou on 02/17/2020
Application: SDS-PAGE Control
SDS-PAGE Control Recombinant Mouse E-Selectin/CD62E Fc Chimera Protein, CF 575-ES
Recombinant Mouse E-Selectin/CD62E Fc Chimera Protein, CF
By Anonymous on 10/27/2018
Application: Binding assay/Protein-protein interaction
Reason for Rating: This product works excellently at 10 ug/mL to detect operable E-selectin cell-surface ligands on cell lines in a quick and convenient flow assay.

Used product at 10 ug/mL or 1 ug/mL with a biolegend anti-human-PE antibody by flow cytometry. Refer to published protocols from Vestweber, Borsig, Dimitroff or others for full protocol.

Binding assay/Protein-protein interaction Recombinant Mouse E-Selectin/CD62E Fc Chimera Protein, CF 575-ES