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Recombinant Mouse CXCL9/MIG Protein

Catalog #: 492-MM
10 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
492-MM-010/CF
492-MM-050/CF

With Carrier

Catalog # Availability Size / Price Qty
492-MM-010
492-MM-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (10)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CXCL9/MIG Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract human peripheral blood lymphocytes (PBL) cultured in the presence of IL-2 for 21 days. The ED50 for this effect is 0.1‑0.3 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CXCR3. The ED50 for this effect is 0.1 - 0.5 µg/mL.
Source
E. coli-derived mouse CXCL9/MIG protein
Thr22-Thr126
Accession #
P18340
N-terminal Sequence
Analysis
Thr22
Predicted Molecular Mass
12 kDa

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492-MM (with carrier)

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SDS

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492-MM/CF (carrier free)

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COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

492-MM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

492-MM/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL9/MIG

CXCL9, also known as MIG, is a member of the alpha  subfamily of chemokines that lacks the ELR domain, and was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse CXCL9 cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The mouse CXCL9 cDNA encodes a 126 amino acid residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield a 105 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes. The E. coli-expressed CXCL9 produced at R&D Systems has been shown to contain greater than 80% full length CXCL9.

References
  1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
  2. Liao, F. et al. (1995) J. Exp. Med. 182:1301.
  3. Vanguri, P. (1995) J. Neuroimmunol. 56:35.
Entrez Gene IDs
4283 (Human); 17329 (Mouse); 246759 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; CXCL9; Gamma-interferon-induced monokine; Humig; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma

Citations for Recombinant Mouse CXCL9/MIG Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. PD-1 combination therapy with IL-2 modifies CD8+ T cell exhaustion program
    Authors: M Hashimoto, K Araki, MA Cardenas, P Li, RR Jadhav, HT Kissick, WH Hudson, DJ McGuire, RC Obeng, A Wieland, J Lee, DT McManus, JL Ross, SJ Im, J Lee, JX Lin, B Hu, EE West, CD Scharer, GJ Freeman, AH Sharpe, SS Ramalingam, A Pellerin, V Teichgräbe, WJ Greenleaf, C Klein, JJ Goronzy, P Umaña, WJ Leonard, KA Smith, R Ahmed
    Nature, 2022-09-28;610(7930):173-181.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Inhibition of the CXCL9-CXCR3 axis suppresses the progression of experimental apical periodontitis by blocking macrophage migration and activation
    Authors: T Hasegawa, V Venkata Su, Y Yahata, M Nakano, S Suzuki, S Suzuki, S Yamada, H Kitaura, I Mizoguchi, Y Noiri, K Handa, M Saito
    Scientific Reports, 2021-01-28;11(1):2613.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Hepatectomy leads to loss of TRAIL-expressing liver NK cells via downregulation of the CXCL9-CXCR3 axis in mice
    Authors: T Yano, M Ohira, R Nakano, Y Tanaka, H Ohdan
    PLoS ONE, 2017-10-31;12(10):e0186997.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. A role for cathepsin Z in neuroinflammation provides mechanistic support for an epigenetic risk factor in multiple sclerosis
    Authors: ERO Allan, RI Campden, BW Ewanchuk, P Tailor, DR Balce, NT McKenna, CJ Greene, AL Warren, T Reinheckel, RM Yates
    J Neuroinflammation, 2017-05-10;14(1):103.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Optimal CD4 T cell priming after LPS-based adjuvanticity with CD134 costimulation relies on CXCL9 production
    Authors: P Shinde, W Liu, A Ménoret, AD Luster, AT Vella
    J. Leukoc. Biol., 2017-04-21;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Murine liver-resident group 1 innate lymphoid cells regulate optimal priming of anti-viral CD8+ T cells
    J Leukoc Biol, 2016-08-04;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. The role of some chemokines from the CXC subfamily in a mouse model of diabetic neuropathy.
    Authors: Zychowska M, Rojewska E, Pilat D, Mika J
    J Diabetes Res, 2015-02-19;2015(0):750182.
    Species: Mouse
    Sample Types: In Vivo
  8. CXCR3 promotes plaque formation and behavioral deficits in an Alzheimer's disease model.
    Authors: Krauthausen M, Kummer M, Zimmermann J, Reyes-Irisarri E, Terwel D, Bulic B, Heneka M, Muller M
    J Clin Invest, 2014-12-15;125(1):365-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Early differentiated CD138(high) MHCII+ IgG+ plasma cells express CXCR3 and localize into inflamed kidneys of lupus mice.
    Authors: Lacotte S, Decossas M, Le Coz C, Brun S, Muller S, Dumortier H
    PLoS ONE, 2013-03-08;8(3):e58140.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Chemokine receptor CXCR3 promotes growth of glioma.
    Authors: Liu C, Luo D, Reynolds BA
    Carcinogenesis, 2010-11-03;32(2):129-37.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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