Recombinant Mouse CLEC4E Fc Chimera Protein, CF Summary
Product Specifications
| MDP | Mouse IgG2A (Glu98-Lys330) | IEGR | Mouse CLEC4E (Thr46-Asp214) Accession # Q9R0Q8 |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9238-CL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Trehalose 6, 6'-Dimycolate is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse CLEC4E Fc Chimera (Catalog # 9238-CL) binds with an ED50 of 8-48 ng/mL.
Reconstitution Calculator
Background: CLEC4E
CLEC4E, also known as Mincle, is an approximately 30 kDa type 2 transmembrane C-type lectin that functions as an activating innate immune receptor (1). Mouse CLEC4E consists of a 19 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 174 aa extracellular domain (ECD) that contains the C-type lectin domain (2). Within the ECD, mouse CLEC4E shares 65% and 87% aa sequence identity with human and rat CLEC4E, respectively. CLEC4E is expressed on monocytes, macrophages, and immature dendritic cells (2-5). It associates with CLEC4D/MCL and the gamma chain signaling subunits of Fc receptors (mediated by an Arg residue in the CLEC4E transmembrane segment) (3, 5, 6). Human CLEC4E binds to mycobacterial glycolipids including the immune adjuvant TDM (cord factor), its synthetic analog TDB, and GroMM (3, 4, 7-10). It also binds the nuclear protein SAP130 which can be released from necrotic cells (5) and cholesterol crystals deposited in atherosclerotic plaques (11). Mouse CLEC4E, in contrast, does not appear to interact with TDB, GroMM, or cholesterol crystals (7, 8, 11). CLEC4E ligation triggers phagocytosis and the production of inflammatory chemokines and cytokines (3-6, 8, 10). The fungus Fonsecaea monophora may evade immune clearance through binding to CLEC4E and suppressing IL-12 production and Th1 cell differentiation instead of promoting inflammation (9).
- Richardson, M.B. and S.J. Williams (2014) Front. Immunol. 5:288.
- Matsumoto, M. et al. (1999) J. Immunol. 163:5039.
- Ishikawa, E. et al. (2009) J. Exp. Med. 206:2879.
- Wells, C.A. et al. (2008) J. Immunol. 180:7404.
- Yamasaki, S. et al. (2008) Nat. Immunol. 9:1179.
- Lobato-Pascual, A. et al. (2013) Eur. J. Immunol. 43:3167.
- Hattori, Y. et al. (2014) J. Biol. Chem. 289:15405.
- Ostrop, J. et al. (2015) J. Immunol. 195:2417.
- Wevers, B.A. et al. (2014) Cell Host Microbe 15:494.
- Ishikawa, T. et al. (2013) Cell Host Microbe 13:477.
- Kiyotake, R. et al. (2015) J. Biol. Chem. 290:25322.
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