Recombinant Mouse CD96 Protein Fc Chimera, CF

Recombinant Mouse CD96 Protein Fc Chimera, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: CD96

CD96, also known as Tactile (T cell-activated increased late expression), is a 160 kDa type I transmembrane glycoprotein of the Ig superfamily that shows peak expression 6-9 days after activation of T, NK, and a subpopulation of B cells (1, 2). The mature extracellular domain (ECD) of CD96 contains three highly N- and O‑glycosylated Ig-like domains, with the two N-terminal domains being V-type and the distal domain a C-type structure (1). Within the mature ECD, mouse CD96 shares 79% and 53% amino acid (aa)sequence identity with rat and human CD96, respectively. In human, a splice variant with a 16 aa deletion in the second V-type domain, called CD96v2, can be expressed (2). CD96 is also frequently expressed on acute myeloid leukemia and myelodysplastic stem cells (3, 4). It is expressed on CD4+ and CD8+ T cells, plus NK cells and select B cells (5). Low expression of adhesive human CD96 is a rare cause of C syndrome, a set of developmental anomalies in cranial bone (trigonocephaly), skin and viscera, demonstrating a role for CD96 in development of these tissues (2, 6). An 80 kDa soluble form is elevated in human serum during chronic hepatitis B (9). The most N-terminal Ig-like domain of human CD96 binds to CD155/PVR (poliovirus receptor), but CD96/CD155 interaction is species-specific (2, 7, 10). On NK cells, co-stimulatory CD96 and DNAM-1 (CD226) are thought to have paired activity with inhibitory TIGIT, all of which bind CD155 and various nectins (11, 12). CD96 can promote NK cell adhesion to, and killing of target cells, including tumors that express CD155 (10, 11).

Related Research Areas

• Acute Myeloid Leukemia
• Cancer Biomarkers
• Cancer Stem Cell Markers
• Co-stimulatory and Co-inhibitory Molecules
• Leukocyte Receptors/Markers in Allergy and Asthma
• Nectin and Nectin-like Binding Receptors and Ligands
• Search Immune Checkpoint Targets