Recombinant Mouse CD90/Thy1 Fc Chimera Protein, CF

Name: Recombinant Mouse CD90/Thy1 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 7335-CD
Rating: 5 (7 reviews)

Catalog #: 7335-CD
2 Citations 7 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7335-CD-050

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Citations (2)

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Reviews (7)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CD90/Thy1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of human neutrophils. The ED₅₀ for this effect is 0.5-3 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse CD90/Thy1 protein

Mouse CD90/Thy1 (Gln20-Lys130) Accession # P01831	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

P01831

N-terminal Sequence
Analysis

No results obtained, Gln20 predicted. Protein identity confirmed by MS analysis of tryptic fragments.

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

40 kDa (monomer)

SDS-PAGE

50-60 kDa, reducing conditions

Product Datasheets

7335-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7335-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CD90/Thy1

CD90, also known as Thy1, is an approximately 25-35 kDa glycoprotein that mediates cellular adhesion and exerts wide ranging effects in different tissues (1, 2). Mature mouse CD90 contains one immunoglobulin-like domain followed by a GPI anchor (3-5). CD90 is expressed on hematopoietic progenitor cells, neurons, and activated vascular endothelial cells (6-8). Its species-specific expression in lymphoid cells allows its use as a thymocyte and pan T cell marker in mouse but not in human (1). CD90 can form dimers and higher order multimers (9). It binds to heparin, the proteoglycan Syndecan-4, and Integrins alpha M beta 2, alpha V beta 3, and alpha V beta 5 (8, 10-13). Through these interactions, CD90 inhibits neurite outgrowth (7), promotes astrocyte adhesion (10), mediates the adhesion and extravasation of leukocytes and melanoma cells (8, 12), supports the Thrombospondin-1 induced disassembly of fibroblast focal adhesions (14), and inhibits the activation of latent TGF-beta 1, myofibroblast differentiation, and lung fibrosis (13)

References

Haeryfar, S.M.M. and D.W. Hoskin (2004) J. Immunol. 173:3581.
Rege, T.A. and J.S. Hagood (2006) Biochim. Biophys. Acta 1763:991.
Chang, H.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82:3819.
Giguere, V. et al. (1985) EMBO J. 4:2017.
Thomas, P.M. and L.E. Samelson (1992) J. Biol. Chem. 267:12317.
Craig, W. et al. (1993) J. Exp. Med. 177:1331.
Tiveron, M-C. et al. (1992) Nature 355:745.
Wetzel, A. et al. (2004) J. Immunol. 172:3850.
Mahanthappa, N.K. and P.H. Patterson (1992) Dev. Biol. 150:60.
Avalos, A.M. et al. (2009) J. Cell Sci. 122:3462.
Hueber, A.O. et al. (1992) J. Immunol. 148:3692.
Saalbach, A. et al. (2005) Oncogene 24:4710.
Zhou, Y. et al. (2010) J. Biol. Chem. 285:22382.
Barker, T.H. et al. (2004) J. Biol. Chem. 279:23510.

Entrez Gene IDs

7070 (Human); 21838 (Mouse)

Alternate Names

CD90 antigen; CD90; CDw90; FLJ33325; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 membrane glycoprotein; Thy-1 T-cell antigen; Thy1

Related Research Areas

Citations for Recombinant Mouse CD90/Thy1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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An alphavbeta3 integrin checkpoint is critical for efficient TH2 cell cytokine polarization and potentiation of antigen-specific immunity
Authors: ACH Szeto, ACF Ferreira, J Mannion, PA Clark, M Sivasubram, MWD Heycock, A Crisp, HE Jolin, P Kozik, MD Knolle, ANJ McKenzie
Nature Immunology, 2022-12-22;24(1):123-135.
Species: Mouse
Sample Types: Beads
Applications: Bioassay
Thy1-Targeted Microbubbles for Ultrasound Molecular Imaging of Pancreatic Ductal Adenocarcinoma
Authors: L Abou-Elkac, H Wang, SM Chowdhury, RH Kimura, SV Bachawal, SS Gambhir, L Tian, JK Willmann
Clin. Cancer Res., 2018-01-04;0(0):.
Species: Mouse
Sample Types: Microbubbles
Applications: Flow Cytometry