Recombinant Mouse CD79B Protein, CF

Catalog #: 9686-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9686-CD-050

Product Details

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CD79B Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse CD79B protein
Val26-Asp158, with a C-terminal 6-His tag

Accession #

P15530

N-terminal Sequence
Analysis

Met29 & Ser31

Predicted Molecular Mass

16 kDa

SDS-PAGE

20-42 kDa, reducing conditions

Product Datasheets

9686-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9686-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CD79B

CD79B (also known as B29, Ig beta and B cell antigen receptor complex-associated protein beta-chain) is a 37-39 kDa member of the Ig-Superfamily. It is expressed on B cells, and forms a covalent heterodimer with CD79a. This complex interacts non-covalently with membrane Ig, forming the B cell antigen receptor. Within this complex, membrane Ig detects antigen while CD79AB initiates signaling (1). CD79B is also required for formation of pre-B cells during B cell development. Mature mouse CD79B is a 203 amino acid (aa) type I transmembrane glycoprotein (aa 26-228). It contains an extracellular region with one V-type Ig-like domain (aa 41-132) and an ITAM‑containing cytoplasmic domain (aa 184-212) (2). Mouse CD79a and CD79B share only 24% aa identity. Over aa 29-158, mouse CD79B shares 54% and 78% aa identity with human and rat CD79B, respectively.

References

Tseng, J. et al. (1997). Blood 89:1513.
Radaev, Sergei, et al. (2010) Structure 18:934.

Entrez Gene IDs

974 (Human); 15985 (Mouse); 171055 (Rat); 102145117 (Cynomolgus Monkey)

Alternate Names

B29; B29B-cell-specific glycoprotein B29; B-cell antigen receptor complex-associated protein beta chain; CD79B antigen (immunoglobulin-associated beta); CD79b antigen; CD79b molecule, immunoglobulin-associated beta; CD79B; IGB; IGBAGM6; Ig-beta; Immunoglobulin-associated B29 protein

Related Research Areas

B Cell Activation

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Mouse CD79B Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse CD79B Protein, CF and earn rewards!

Have you used Recombinant Mouse CD79B Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review