Recombinant Mouse CD6 His Tagged Protein, CF

Recombinant Mouse CD6 His Tagged Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: CD6

CD6 is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, which is characterized by the presence of one or several repeats of SRCR domains in their extracellular region (1). CD6 is a type I transmembrane glycoprotein and contains three extracellular SRCR domains (2, 3). It is expressed on thymocytes, T cells, a subset of B cells, and on certain regions of the brain (3, 4). Mouse CD6 is a 665 amino acid (aa) protein that includes a 16 aa signal sequence, a 382 aa extracellular domain, a 21 aa transmembrane segment, and a 246 aa cytoplasmic region (5, 6). Within the ECD, mouse CD6 shares 69% and 88% aa sequence identity with human and rat CD6, respectively. CD6 appears to play a role as both a co-stimulatory molecule in T cell activation and as an adhesion receptor. Studies demonstrating a mitogenic effect for T cells with some CD6-specific monoclonal antibodies, in conjunction with either accessory cells or PMA and anti-CD2 mAb, support the concept of CD6 as a co-stimulatory molecule (7-12). Additionally, anti-CD6 monoclonal antibody has been used as an immunosuppressive agent for patients undergoing kidney or bone marrow allograft rejection. It has also been used to remove CD6⁺ T cells from donor bone marrow prior to allogenic bone marrow transplantation. Other studies have demonstrated an adhesive role for CD6. It has been demonstrated to bind the activated leukocyte cell adhesion molecule (ALCAM, CD166). CD6/ALCAM interactions have been postulated to play a role in thymocyte development (9, 13). Additionally, the presence of ALCAM on neuronal cells may provide a mechanism of interaction between CD6⁺ T cell and ALCAM⁺ neuronal cells. Phosphorylation of the CD6 molecule appears to play a role in CD6-mediated signal transduction (9, 13). Serine and threonine residues become hyperphosphorylated and tyrosine residues become phosphorylated when T cells are activated with anti-CD6 mAb in conjunction with PMA, anti-CD2, or anti-CD3 mAb (8, 10, 11, 14). The CD6 intracellular domain contains regions that can interact with SH2 or SH3-containing proteins. However, the signaling pathways have not been elucidated (5, 15, 16).

Related Research Areas

• ITIM/ITAM Immunoreceptors and Related Molecules
• Leukocyte Receptors/Markers in Allergy and Asthma
• Scavenger Receptors
• T Cell Migration/Adhesion