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Recombinant Mouse CD200R1L Fc Chimera Protein, CF

Catalog #: 10198-CD Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10198-CD-050
Recombinant Mouse CD200R1L Fc Chimera Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CD200R1L Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse CD200R1L Fc Chimera (Catalog # 10198-CD) binds with an ED50 of 0.35-2.8 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CD200R1L protein
Mouse CD200R1L
(Ile25-Thr220)
Accession # Q6XJV6.1		IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
Q6XJV6.1
N-terminal Sequence
Analysis
Ile25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
48 kDa
SDS-PAGE
65-85 kDa, under reducing conditions

Product Datasheets

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10198-CD

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10198-CD

Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Binding Activity Recombinant Mouse CD200R1L Fc Chimera Protein Binding Activity View Larger

When Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse CD200R1L Fc Chimera (Catalog # 10198-CD) binds with an ED50 of 0.35-2.8 µg/mL.

SDS-PAGE Recombinant Mouse CD200R1L Fc Chimera Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse CD200R1L Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 65-85 kDa and 130-170 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD200R1L

CD200 Receptor 2 (CD200R2), or CD200R1L, is a type I transmembrane receptor for the CD200 cell surface glycoprotein. Mature CD200R2 consists of an extracellular domain (ECD) containing an Ig-like V-type domain and an Ig-like C2-type domain, a transmembrane segment and a short cytoplasmic domain. The ECD of mouse CD200R2 shares a 76% and 63% amino acid (aa) identity with the rat and human ECD, respectively. CD200R2 is strongly expressed on resting mast cells but deceased upon activation via Fc epsilon R1. It is also observed on monocytes, bone marrow-derived dendritic cells and TH2 cells (1, 2, 4). It has been demonstrated CD200R2 can interact with CD200 (3) and signal through adaptor protein, DAP12, via its lysine residue in the transmembrane region (2). In addition, it has been reported CD200 and CD200R2 interaction alters dendritic cell differentiation and enhances induction of CD4+CD25+Foxp3+ regulatory T cells in mouse transplant model (5).

References
  1. Wright, G. et al. (2003) J. Immunol. 171:3034.
  2. Voehringer, D. (2004) J. Biol. Chem. 279(52):54117.
  3. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
  4. Minas, K. (2006) Crit Rev Immunol 26(3):213.
  5. Gorczynski, R. et al. (2008) J. Immunol. 180(9):5946.
Long Name
CD200 Receptor 1 Like
Entrez Gene IDs
344807 (Human); 271375 (Mouse); 501779 (Rat)
Alternate Names
cell surface glycoprotein CD200 receptor 2; CD200 receptor 1-like; CD200R1L; CD200R2; CD200RLa

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