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Recombinant Mouse CCL3/MIP-1 alpha Protein

Catalog #: 450-MA
31 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
450-MA-010/CF
450-MA-050/CF

With Carrier

Catalog # Availability Size / Price Qty
450-MA-010
450-MA-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (31)
FAQs
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CCL3/MIP-1 alpha Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract 2-day cultured human monocytes. The ED50 for this effect is 5‑25 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5. The ED50 for this effect is 0.4‑2.0 ng/mL.
Source
E. coli-derived mouse CCL3/MIP-1 alpha protein
Ala24-Ala92
Accession #
Q5QNW0
N-terminal Sequence
Analysis
Ala24
Predicted Molecular Mass
7.8 kDa

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450-MA (with carrier)

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450-MA/CF (carrier free)

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COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

450-MA

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

450-MA/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL3/MIP-1 alpha

CCL3, also known as macrophage inflammatory protein 1 alpha (MIP-1 alpha ) and LD78, is a member of the beta or CC subfamily of chemokines and is closely related to CCL4/MIP-1 beta. Chemokines comprise a large family of small secreted proteins that are involved in immune and inflammatory responses. CCL3 expression can be induced in a variety of hematopoietic cells, fibroblasts, smooth muscle cells, and epithelial cells (1). Mature mouse CCL3 shares 73%, 91%, and 82% amino acid sequence identity with human, rat, and cotton rat CCL3, respectively (2). CCL3 is an approximately 8 kDa chemokine that forms complexes with sulfated proteoglycans (3, 4). In a reversible process, CCL3 associates into noncovalently-linked dimers which then form tetramers and high molecular weight polymers (5, 6). These complexes of CCL3 are protected from proteolytic digestion by insulin degrading enzyme (IDE) which can cleave the monomeric chemokine (6). CCL3 exerts its biological functions through interactions with CCR1, CCR3, and CCR5 (1). It is cleared from the extracellular space by internalization via the decoy chemokine receptor D6 (7). CCL3 promotes the chemoattraction, adhesion to activated vascular endothelium, and cellular activation of many hematopoietic cell types including activated T cells, NK cells, neutrophils, monocytes, immature dendritic cells, and eosinophils (1, 8 - 10). CCL3 is also known as stem cell inhibitor (SCI) and can inhibit the proliferation of hematopoietic progenitor cells (3). CCL3 bioactivity contributes to tumor metastasis and the inflammatory components of viral infection, rheumatoid arthritis, and hepatitis (11 - 14), although it also can suppress the replication of HIV (15). CCL3 additionally promotes hyperalgesia by sensitizing sensory neurons to TRPV1-mediated noxious stimulation (16).

References
  1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
  2. Davatelis, G. et al. (1988) J. Exp. Med. 167:1939.
  3. Graham, G.J. et al. (1990) Nature 344:442.
  4. Wagner, L. et al. (1998) Nature 391:908.
  5. Graham, G.J. et al. (1994) J. Biol. Chem. 269:4974.
  6. Ren, M. et al. (2010) EMBO J. 29:3952.
  7. Weber, M. et al. (2004) Mol. Biol. Cell 15:2492
  8. Taub, D.D. et al. (1993) Science 260:355.
  9. Bernardini, G. et al. (2008) Blood 111:3626.
  10. Lee, S.C. et al. (2000) J. Immunol. 164:3392.
  11. Wu, Y. et al. (2008) J. Immunol. 181:6384.
  12. Cook, D.N. et al. (1995) Science 269:1583.
  13. Chintalacharuvu, S.R. et al. (2005) Immunol. Lett. 100:202.
  14. Ajuebor, M.N. et al. (2004) Eur. J. Immunol. 34:2907.
  15. Cocchi, F. et al. (1995) Science 270:1811.
  16. Zhang, N. et al. (2005) Proc. Natl. Acad. Sci. 102:4536.
Entrez Gene IDs
6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine); 102136134 (Cynomolgus Monkey)
Alternate Names
C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; MIP1A; MIP-1alpha; MIP1-alpha; PAT 464.1; SCYA3; SIS-beta

Citations for Recombinant Mouse CCL3/MIP-1 alpha Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. Overriding impaired FPR chemotaxis signaling in diabetic neutrophil stimulates infection control in murine diabetic wound
    Authors: R Roy, J Zayas, SK Singh, K Delgado, SJ Wood, MF Mohamed, DM Frausto, YA Albalawi, TP Price, R Estupinian, EF Giurini, TM Kuzel, A Zloza, J Reiser, SH Shafikhani
    Elife, 2022-02-03;11(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Distinct transcription factor networks control neutrophil-driven inflammation
    Authors: TE Khoyratty, Z Ai, I Ballestero, HL Eames, S Mathie, S Martín-Sal, L Wang, A Hemmings, N Willemsen, V von Werz, A Zehrer, B Walzog, E van Grinsv, A Hidalgo, IA Udalova
    Nature Immunology, 2021-07-19;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Prevention of CaCl2-induced aortic inflammation and subsequent aneurysm formation by the CCL3-CCR5 axis
    Authors: Y Ishida, Y Kuninaka, M Nosaka, A Kimura, A Taruya, M Furuta, N Mukaida, T Kondo
    Nat Commun, 2020-11-25;11(1):5994.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity
    Authors: S Sharif, N Ueda, Y Nakatani, LM Wise, S Clifton, Z Lateef, AA Mercer, SB Fleming
    Front Microbiol, 2019-06-25;10(0):1421.
    Species: Mouse
    Sample Types:
    Applications: Binding Assay
  5. Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling
    Authors: ML Capitano, N Mor-Vaknin, AK Saha, S Cooper, M Legendre, H Guo, R Contreras-, F Kappes, MA Sartor, CT Lee, X Huang, DM Markovitz, HE Broxmeyer
    J. Clin. Invest., 2019-05-20;130(0):2555-2570.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Relevance of CCL3/CCR5 axis in oral carcinogenesis
    Authors: JM da Silva, TP Moreira Do, LM Sobral, CM Queiroz-Ju, MA Rachid, AEI Proudfoot, GP Garlet, AC Batista, MM Teixeira, AM Leopoldino, RC Russo, TA Silva
    Oncotarget, 2017-04-06;8(31):51024-51036.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  7. CD84 mediates CLL-microenvironment interactions
    Oncogene, 2016-07-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Activation of phagocytic activity in astrocytes by reduced expression of the inflammasome component ASC and its implication in a mouse model of Alzheimer disease.
    Authors: Couturier J, Stancu I, Schakman O, Pierrot N, Huaux F, Kienlen-Campard P, Dewachter I, Octave J
    J Neuroinflammation, 2016-01-27;13(1):20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Hyaluronan Binding Identifies a Functionally Distinct Alveolar Macrophage-like Population in Bone Marrow-Derived Dendritic Cell Cultures.
    Authors: Poon G, Dong Y, Marshall K, Arif A, Deeg C, Dosanjh M, Johnson P
    J Immunol, 2015-06-17;195(2):632-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Murine IL-17+ Vgamma4 T lymphocytes accumulate in the lungs and play a protective role during severe sepsis.
    Authors: Costa M, de Negreiros C, Bornstein V, Valente R, Mengel J, Henriques M, Benjamim C, Penido C
    BMC Immunol, 2015-06-03;16(0):36.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Tetanus toxoid and CCL3 improve dendritic cell vaccines in mice and glioblastoma patients.
    Authors: Mitchell, Duane A, Batich, Kristen, Gunn, Michael, Huang, Min-Nung, Sanchez-Perez, Luis, Nair, Smita K, Congdon, Kendra L, Reap, Elizabet, Archer, Gary E, Desjardins, Annick, Friedman, Allan H, Friedman, Henry S, Herndon, James E, Coan, April, McLendon, Roger E, Reardon, David A, Vredenburgh, James J, Bigner, Darell D, Sampson, John H
    Nature, 2015-03-11;519(7543):366-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  12. The Friend leukaemia virus integration 1 (Fli-1) transcription factor affects lupus nephritis development by regulating inflammatory cell infiltration into the kidney.
    Authors: Sato S, Zhang X
    Clin Exp Immunol, 2014-07-01;177(1):102-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Macrophage-inducing FasL on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration.
    Authors: Fujihara Y, Takato T, Hoshi K
    Stem Cells, 2014-05-01;32(5):1208-19.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Endothelial cell-derived chemerin promotes dendritic cell transmigration.
    Authors: Gonzalvo-Feo S, Del Prete A, Pruenster M, Salvi V, Wang L, Sironi M, Bierschenk S, Sperandio M, Vecchi A, Sozzani S
    J Immunol, 2014-01-27;192(5):2366-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. CCR1 inhibition ameliorates the progression of lupus nephritis in NZB/W mice.
    Authors: Bignon A, Gaudin F, Hemon P, Tharinger H, Mayol K, Walzer T, Loetscher P, Peuchmaur M, Berrebi D, Balabanian K
    J Immunol, 2013-12-23;192(3):886-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. C-C motif chemokine CCL3 and canonical neutrophil attractants promote neutrophil extravasation through common and distinct mechanisms.
    Authors: Reichel CA, Puhr-Westerheide D, Zuchtriegel G, Uhl B, Berberich N, Zahler S, Wymann MP, Luckow B, Krombach F
    Blood, 2012-06-06;120(4):880-90.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  17. Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
    Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
    Am. J. Pathol., 2011-10-01;179(6):2730-9.
    Applications: ELISA (Standard)
  18. TLR2-mediated production of IL-27 and chemokines by respiratory epithelial cells promotes bleomycin-induced pulmonary fibrosis in mice.
    Authors: Kim HS, Go H, Akira S, Chung DH
    J. Immunol., 2011-09-19;187(8):4007-17.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  19. Estrogen-dependent and C-C chemokine receptor-2-dependent pathways determine osteoclast behavior in osteoporosis.
    Authors: Binder NB, Niederreiter B, Hoffmann O, Stange R, Pap T, Stulnig TM, Mack M, Erben RG, Smolen JS, Redlich K
    Nat. Med., 2009-03-29;15(4):417-24.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
    Authors: Wu Y, Li YY, Matsushima K, Baba T, Mukaida N
    J. Immunol., 2008-11-01;181(9):6384-93.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Gelatinases mediate neutrophil recruitment in vivo: evidence for stimulus specificity and a critical role in collagen IV remodeling.
    Authors: Reichel CA, Rehberg M, Bihari P, Moser CM, Linder S, Khandoga A, Krombach F
    J. Leukoc. Biol., 2008-01-03;83(4):864-74.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  22. Bv8 regulates myeloid-cell-dependent tumour angiogenesis.
    Authors: Shojaei F, Wu X, Zhong C, Yu L, Liang XH, Yao J, Blanchard D, Bais C, Peale FV, van Bruggen N, Ho C, Ross J, Tan M, Carano RA, Meng YG, Ferrara N
    Nature, 2007-12-06;450(7171):825-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Involvement of CC chemokines in gammadelta T lymphocyte trafficking during allergic inflammation: the role of CCL2/CCR2 pathway.
    Authors: Penido C, Costa MF, Souza MC, Costa KA, Candea AL, Benjamim CF, Henriques MG
    Int. Immunol., 2007-12-04;20(1):129-39.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  24. Dendritic cell transmigration through brain microvessel endothelium is regulated by MIP-1alpha chemokine and matrix metalloproteinases.
    Authors: Zozulya AL, Reinke E, Baiu DC, Karman J, Sandor M, Fabry Z
    J. Immunol., 2007-01-01;178(1):520-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
    Authors: Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
    Eur. J. Immunol., 2006-08-01;36(8):2025-34.
    Applications: ELISA (Standard)
  26. Immunosuppressant triptolide inhibits dendritic cell-mediated chemoattraction of neutrophils and T cells through inhibiting Stat3 phosphorylation and NF-kappaB activation.
    Authors: Liu Q, Chen T, Chen G, Li N, Wang J, Ma P, Cao X
    Biochem. Biophys. Res. Commun., 2006-05-12;345(3):1122-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Selective macrophage suppression during sepsis.
    Authors: Ellaban E, Bolgos G, Remick D
    Cell. Immunol., 2005-02-26;231(1):103-11.
    Applications: ELISA (Standard)
  28. Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration.
    Authors: Perrin FE, Lacroix S, Aviles-Trigueros M, David S
    Brain, 2005-02-02;128(0):854-66.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  29. Novel chemokine responsiveness and mobilization of neutrophils during sepsis.
    Authors: Speyer CL, Gao H, Rancilio NJ, Neff TA, Huffnagle GB, Sarma JV, Ward PA
    Am. J. Pathol., 2004-12-01;165(6):2187-96.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  30. A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection.
    Authors: Reading PC, Symons JA, Smith GL
    J. Immunol., 2003-02-01;170(3):1435-42.
    Applications: ELISA (Standard)
  31. Role of the autocrine chemokines MIP-1alpha and MIP-1beta in the metastatic behavior of murine T cell lymphoma.
    Authors: Menten P, Saccani A, Dillen C, Wuyts A, Struyf S, Proost P, Mantovani A, Wang JM, Van Damme J
    J. Leukoc. Biol., 2002-10-01;72(4):780-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Mouse CCL3/MIP-1 alpha Protein
By Anonymous on 11/27/2017
Application: Immunoassay Standard

This protein was used as a calibrator in and ELISA employing antibodies MAB450 and BAF450.

Immunoassay Standard Recombinant Mouse CCL3/MIP-1 alpha Protein 450-MA